Pig plasminogen activator inhibitor 1,PAI-1 ELISA Kit

Pig plasminogen activator inhibitor 1,PAI-1 ELISA Kit

产品详情

产品评价

靶点详情

产品详情

产品评价

相关产品

靶点详情

Most popular with customers

产品中心

信号通路

技术信息

联系信息

中文名称：

猪纤溶酶原激活物抑制因子1(PAI-1)酶联免疫试剂盒
货号：

CSB-E06534p
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情
产品评价
相关产品
靶点详情

产品描述：

CUSABIO猪纤溶酶原激活物抑制因子1（PAI-1）酶联免疫检测试剂盒（货号CSB-E06534p），基于双抗体夹心法定量检测血清、血浆及组织匀浆样本中的PAI-1水平。PAI-1是一种丝氨酸蛋白酶抑制剂，通过抑制纤溶酶原激活物（如tPA和uPA）调控纤维蛋白溶解系统，在血栓形成、组织纤维化、炎症反应及细胞外基质重塑等生理病理过程中发挥重要作用。试剂盒检测灵敏度为0.312 ng/mL，线性范围覆盖0.312-20 ng/mL，可精准检测微量样本中PAI-1的表达变化。该试剂盒适用于心血管疾病模型、代谢综合征研究、创伤修复机制探索等科研领域，为分析PAI-1在凝血-纤溶系统失衡、器官纤维化或肿瘤微环境中的作用提供可靠工具。其标准化操作流程与高特异性抗体组合确保实验结果的重复性与准确性，满足基础研究中对PAI-1蛋白动态变化的定量分析需求。
别名：

SERPINE1 ELISA Kit; PAI1 ELISA Kit; PLANH1 ELISA Kit; Plasminogen activator inhibitor 1 ELISA Kit; PAI ELISA Kit; PAI-1 ELISA Kit; Endothelial plasminogen activator inhibitor ELISA Kit; Serpin E1 ELISA Kit
缩写：

SERPINE1
Uniprot No.：

P79335
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.312 ng/mL-20 ng/mL
灵敏度：

0.078 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cell Biology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig PAI-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	83-93
1:2	Average %	100
	Range %	95-104
1:4	Average %	90
	Range %	85-94
1:8	Average %	95
	Range %	89-100

回收率：

The recovery of pig PAI-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	87-97
EDTA plasma (n=4)	98	90-102

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.050	2.012	2.031	1.905
10	1.669	1.676	1.673	1.547
5	1.397	1.366	1.382	1.256
2.5	0.996	0.987	0.992	0.866
1.25	0.668	0.678	0.673	0.547
0.625	0.464	0.457	0.461	0.335
0.312	0.303	0.299	0.301	0.175
0	0.125	0.127	0.126

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

Serine protease inhibitor. Inhibits TMPRSS7. Is a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). As PLAT inhibitor, it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots. As PLAU inhibitor, it is involved in the regulation of cell adhesion and spreading. Acts as a regulator of cell migration, independently of its role as protease inhibitor. It is required for stimulation of keratinocyte migration during cutaneous injury repair. It is involved in cellular and replicative senescence. Plays a role in alveolar type 2 cells senescence in the lung. Is involved in the regulation of cementogenic differentiation of periodontal ligament stem cells, and regulates odontoblast differentiation and dentin formation during odontogenesis.
基因功能参考文献：
1. In one pig population and in female pigs, SERPINE1 (serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1) was significantly associated with muscling, growth and fat accretion and in male animals with meat quality. PMID: 22497544
亚细胞定位：

Secreted.
蛋白家族：

Serpin family
数据库链接：

KEGG: ssc:396945

UniGene: Ssc.9781

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.