Pig monocyte chemotactic protein 1,MCP1 ELISA Kit

Pig monocyte chemotactic protein 1,MCP1 ELISA Kit

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中文名称：

猪单核细胞趋化蛋白1(MCP-1/CCL2/MCAF)酶联免疫试剂盒
货号：

CSB-E13178p
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO猪单核细胞趋化蛋白1（MCP-1/CCL2/MCAF）酶联免疫检测试剂盒，基于双抗体夹心法原理实现定量分析，适用于科研领域中对猪血清、血浆样本中MCP-1蛋白含量的精准检测。MCP-1属于CC趋化因子家族，通过与CCR2受体结合介导单核细胞、巨噬细胞迁移，在炎症反应、免疫调节及组织损伤修复中发挥关键作用。本试剂盒检测范围为31.25-2000 pg/mL，最低检测灵敏度达31.25 pg/mL，采用预包被特异性抗体的96孔板，通过标准品梯度稀释建立定量曲线，结合生物素标记检测抗体与链霉亲和素-HRP显色系统，可实现高特异性、高重复性的靶蛋白定量。该试剂盒广泛应用于动物疾病模型研究、免疫调控机制探索及炎症相关分子通路分析，例如猪病毒感染、细菌性肺炎等病理过程中单核细胞趋化活性的动态监测。实验操作需使用新鲜采集的血清或EDTA抗凝血浆，避免反复冻融或溶血干扰检测结果，配套提供标准品、检测抗体及显色液等完整组分，支持手动或自动化设备完成检测流程。
别名：

CCL2 ELISA Kit; SCYA2C-C motif chemokine 2 ELISA Kit; Monocyte chemoattractant protein 1 ELISA Kit; Monocyte chemotactic protein 1 ELISA Kit; MCP-1 ELISA Kit; Small-inducible cytokine A2 ELISA Kit
缩写：

CCL2
Uniprot No.：

P42831
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.81 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig MCP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	87-96
1:2	Average %	98
	Range %	93-102
1:4	Average %	85
	Range %	81-89
1:8	Average %	96
	Range %	93-99

回收率：

The recovery of pig MCP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	88-96
EDTA plasma (n=4)	85	80-90

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.078	2.069	2.074	1.880
1000	1.610	1.687	1.649	1.455
500	1.099	1.059	1.079	0.885
250	0.615	0.638	0.627	0.433
125	0.427	0.438	0.433	0.239
62.5	0.342	0.349	0.346	0.152
31.25	0.276	0.268	0.272	0.078
0	0.189	0.198	0.194

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Probiotics-fermented Massa Medicata Fermentata ameliorates weaning stress in piglets related to improving intestinal homeostasis Yanbo Wang, et al,Applied Microbiology and Biotechnology,2018
Effects of dietary Bacillus amyloliquefaciens supplementation on growth performance, intestinal morphology, inflammatory response, and microbiota of intra Yue Li.et al,Journal of Animal Science and Biotechnology,2018
Sevoflurane anesthetic preconditioning protects the lung endothelial glycocalyx from ischemia reperfusion injury in an experimental lung autotransplant model. Casanova J.et al,J Anesth.,2016

功能：

Acts as a ligand for C-C chemokine receptor CCR2. Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions. Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils. Plays an important role in mediating peripheral nerve injury-induced neuropathic pain. Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B.
基因功能参考文献：
1. these results provide novel insights into CCL2-mediated signaling mechanisms in the porcine endometrium at the maternal-fetal interface during early pregnancy PMID: 29278679
2. CXCL10 protein was detected in serum from pigs infected with a high virulence African swine fever virus isolate. Levels of CCL2 mRNA were increased in pigs infected with the high virulence isolate compared to a low virulence isolate. PMID: 24083897
3. Porcine CCL2 binding on Tuk4+SLA-II- but not on Tuk4-SLA-II+ cells was detected by using a CCL2-green fluorescence protein fusion protein. PMID: 20670605
4. The protective properties of flavonoids, such as EGCG, against endothelial inflammation may be regulated in part though induction of HO-1 and subsequent activator protein-1 signaling. PMID: 20580034
5. MCP-1 contributes to functional and structural impairment in the kidney in renal artery stenosis, mainly in the tubulointerstitial compartment PMID: 19730125
6. may contribute to xenograft rejection by attracting monocytes into the islets PMID: 14962280
7. Contrast media did not affect bone marrow cell viability/MCP-1 secretion. PMID: 15274157
8. Porcine vessel wall injury via balloon arthroplasty upregulates expression of CCL2 by coronary artery transmural and perivascular cells in a sequential pattern. PMID: 16926016
9. in the aortic arch expression of monocyte chemoattractant protein 1 (MCP-1) was upregulated, compared with the thoracic aorta PMID: 17588585
10. Variable gene expression (eg, matrix metalloproteinase-9, CCL2 and Lp-PLA(2) mRNAs), both in regard to the arterial bed and duration of disease, was associated with variable plaque development and progression. PMID: 18276914
11. myocardial infarction induced proinflammatory gene and protein expression in peripheral blood mononuclear cells of tissue factor cyclo-oxygenase-2, monocyte chemoattractant protein-1 and CRP PMID: 19036073
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亚细胞定位：

Secreted.
蛋白家族：

Intercrine beta (chemokine CC) family
数据库链接：

KEGG: ssc:397422

STRING: 9823.ENSSSCP00000018779

UniGene: Ssc.657

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.