Pig matrix metalloproteinase 9,MMP-9 ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

本产品是专为科研领域设计的猪基质金属蛋白酶9（MMP-9）定量检测试剂盒（货号：CSB-E06798p），采用双抗体夹心酶联免疫法，可精准检测猪血清、血浆及组织匀浆样本中的MMP-9含量。MMP-9作为基质金属蛋白酶家族成员，在细胞外基质降解、炎症反应及组织重塑等生理病理过程中发挥重要作用。试剂盒检测灵敏度达0.156 ng/mL，线性范围为0.625-40 ng/mL，操作全程约需3小时，配备预包被特异性抗体的96孔板及冻干标准品（需复溶后使用），标准品与检测组分需于-20℃保存，开封后板条需密封冷藏并于1个月内使用。该产品适用于动物模型研究，例如炎症性疾病机制探索、创伤修复过程分析或与MMP-9相关的病理状态评估，可为科研人员提供稳定可靠的定量数据支持，但不可用于临床诊断或其他非科研用途。
缩写：

MMP-9
Uniprot No.：

Q2VI03
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.625 ng/mL-40 ng/mL
灵敏度：

0.156 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cancer
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig MMP-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	85-106
1:2	Average %	95
	Range %	91-99
1:4	Average %	96
	Range %	88-105
1:8	Average %	95
	Range %	90-102

回收率：

The recovery of pig MMP-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	87-100
EDTA plasma (n=4)	97	93-102

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.