Pig matrix metalloproteinase 2/Gelatinase A,MMP-2 ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO猪基质金属蛋白酶2/明胶酶A（MMP-2/Gelatinase A）定量检测试剂盒（货号：CSB-E12842p），基于双抗体夹心法原理设计，可精准检测血清、血浆、细胞培养上清及组织匀浆等多种样本中MMP-2的蛋白浓度，检测灵敏度范围为47-3000 ng/mL。MMP-2作为基质金属蛋白酶家族成员，在细胞外基质降解、组织重塑及病理过程中发挥重要作用，其活性变化与炎症反应、伤口修复及肿瘤微环境调控等生物学过程密切相关。本试剂盒通过特异性抗体捕获与信号放大系统，实现对目标蛋白的高特异性定量分析，适用于科研领域探究猪模型中的组织再生机制、疾病相关分子通路及药物干预效果评估等方向，为深入解析MMP-2在生理病理中的功能提供高效检测方案。试剂盒严格验证批次稳定性，操作流程标准化，配套完整反应组分，确保实验数据的可重复性和准确性。
缩写：

MMP2/Gelatinase A
Uniprot No.：

F1RF11
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

47 ng/mL-3000 ng/mL
灵敏度：

11.75 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cancer
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig MMP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	92-97
1:2	Average %	103
	Range %	99-105
1:4	Average %	93
	Range %	89-98
1:8	Average %	92
	Range %	87-99

回收率：

The recovery of pig MMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-97
EDTA plasma (n=4)	90	86-93

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.