Pig major acute phase protein (MAP) ELISA Kit

Pig major acute phase protein (MAP) ELISA Kit

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中文名称：

猪主要急性期蛋白( MAP)酶联免疫试剂盒
货号：

CSB-E13425p
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO猪主要急性期蛋白（MAP）酶联免疫检测试剂盒（货号：CSB-E13425p），采用竞争法原理实现定量分析，适用于检测猪血清、血浆及组织匀浆样本中的MAP浓度。MAP作为重要的急性期反应蛋白，在机体遭遇炎症、感染或组织损伤时显著升高，其动态变化可反映机体的免疫应答状态，是科研中监测猪群健康状态和疾病进程的重要生物标志物。试剂盒检测范围为37.5-2400 ng/mL，通过优化预包被板与生物素标记检测抗体的特异性结合，实现精准检测：样本中的MAP蛋白与标记物竞争结合抗体，显色强度与目标物浓度呈负相关，最终通过标准曲线计算浓度值。试剂盒包含预包被微孔板、系列浓度标准品、检测抗体、显色液及终止液等完整组件，操作流程标准化。该产品适用于动物免疫学研究、疾病模型建立后的病理分析，以及抗炎药物或疫苗效果评估等科研场景，尤其可为猪的急性炎症反应机制研究、组织损伤修复实验提供定量检测支持。
别名：

ITIH4 ELISA Kit; IHRP ELISA Kit; Inter-alpha-trypsin inhibitor heavy chain H4 ELISA Kit; ITI heavy chain H4 ELISA Kit; ITI-HC4 ELISA Kit; Inter-alpha-inhibitor heavy chain 4 ELISA Kit; Inter-alpha-trypsin inhibitor family heavy chain-related protein ELISA Kit; IHRP ELISA Kit; Major acute phase protein ELISA Kit; MAP ELISA Kit
缩写：

ITIH4
Uniprot No.：

P79263
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

37.5 ng/mL-2400 ng/mL
灵敏度：

9.38 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig MAP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	97
	Range %	92-104
1:400	Average %	95
	Range %	91-99
1:800	Average %	93
	Range %	87-99
1:1600	Average %	86
	Range %	82-92

回收率：

The recovery of pig MAP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-101
EDTA plasma (n=4)	97	90-102

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
2400	0.138	0.141	0.140
1200	0.457	0.460	0.459
600	0.903	0.898	0.901
300	1.134	1.121	1.128
150	1.518	1.483	1.501
75	1.689	1.706	1.698
37.5	2.032	2.049	2.041
0	2.187	2.114	2.151

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Olive oil bioactives protect pigs against experimentally-induced chronic inflammation independently of alterations in gut microbiota Martin Liehr.et al,PLOS ONE,2017

功能：

Type II acute-phase protein (APP) involved in inflammatory responses to trauma. May also play a role in liver development or regeneration.
基因功能参考文献：
1. Promoter of ITIH4 major acute phase protein has been isolated, cloned, and proven functional by response to an inflammatory stimulus (interleukin-6) in human Hep3B cells. (inter-alpha-trypsin heavy chain 4; ITIH4) PMID: 16275952
亚细胞定位：

Secreted.
蛋白家族：

ITIH family
组织特异性：

Liver specific.
数据库链接：

KEGG: ssc:396799

STRING: 9823.ENSSSCP00000012206

UniGene: Ssc.50918

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.