Pig intestinal fatty acid binding protein,iFABP ELISA Kit

Pig intestinal fatty acid binding protein,iFABP ELISA Kit

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中文名称：

猪肠脂肪酸结合蛋白(iFABP)酶联免疫试剂盒
货号：

CSB-E13994p
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO猪肠脂肪酸结合蛋白（iFABP）酶联免疫试剂盒（货号：CSB-E13994p），通过双抗体夹心法定量检测血清或血浆样本中iFABP的表达水平。iFABP是一种主要表达于小肠上皮细胞的胞质蛋白，参与膳食脂肪酸的吸收与转运，其在外周血中的异常升高可作为肠道屏障损伤的重要生物标志物，适用于肠道炎症、缺血再灌注损伤等病理机制的科研分析。该试剂盒检测灵敏度为78 pg/mL，线性范围覆盖78-5000 pg/mL，实验全程可在4.5小时内完成，采用预包被96孔板形式，标准品与样本仅需50 μL即可进行检测，配套提供专属检测缓冲液与终止液。本产品适用于科研场景中动物模型肠道损伤的动态监测、营养干预对肠道功能影响评估，或药物研发中肠道保护效果的量化分析，实验数据可通过酶标仪在450 nm波长下快速读取，为消化道相关疾病的基础研究提供标准化检测方案。
别名：

FABP2 ELISA Kit; FABPIFatty acid-binding protein ELISA Kit; intestinal ELISA Kit; Fatty acid-binding protein 2 ELISA Kit; Intestinal-type fatty acid-binding protein ELISA Kit; I-FABP ELISA Kit
缩写：

FABP2
Uniprot No.：

Q45KW7
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma
检测范围：

78 pg/mL-5000 pg/mL
灵敏度：

19.5 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig iFABP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	87-93
1:2	Average %	100
	Range %	97-103
1:4	Average %	85
	Range %	81-89
1:8	Average %	93
	Range %	90-96

回收率：

The recovery of pig iFABP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	84	81-89
EDTA plasma (n=4)	99	93-103

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
5000	2.129	2.138	2.134	1.976
2500	1.628	1.656	1.642	1.484
1250	1.084	1.087	1.086	0.928
625	0.607	0.638	0.623	0.465
312	0.445	0.426	0.436	0.278
156	0.348	0.323	0.336	0.178
78	0.268	0.251	0.260	0.102
0	0.157	0.159	0.158

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor.
基因功能参考文献：
1. The full-length cDNA of I-FABP was cloned from intestine and the mRNA was extensively present in various tissues, but I-FABP transcript of approximately 620 bp was more abundant in intestine than in other tissues. PMID: 16529296
2. Plasma I-FABP concentration showed great variation within treatments, and no difference was observed in plasma I-FABP concentrations between the weaned conventionally and unweaned treatments PMID: 18708596
3. Association analyses of the FABP2:g.412T>C with fatty acid composition traits were not significant in simple association. PMID: 19207930
亚细胞定位：

Cytoplasm.
蛋白家族：

Calycin superfamily, Fatty-acid binding protein (FABP) family
数据库链接：

KEGG: ssc:595106

STRING: 9823.ENSSSCP00000025576

UniGene: Ssc.16525

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.