Pig interleukin 8,IL-8 ELISA Kit

Pig interleukin 8,IL-8 ELISA Kit

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中文名称：

猪白介素8(IL-8/CXCL8)酶联免疫试剂盒
货号：

CSB-E06787p
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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CUSABIO猪白介素8（IL-8/CXCL8）酶联免疫检测试剂盒（货号CSB-E06787p），采用双抗体夹心法原理实现定量分析，可精准检测猪血清、血浆、细胞培养上清及组织匀浆样本中IL-8/CXCL8的浓度水平。IL-8作为重要的趋化因子，在宿主抵御病原体感染、中性粒细胞趋化及炎症反应调控中发挥核心作用，其动态监测对猪类免疫机制研究和疾病模型建立具有重要科研价值。该试剂盒具备125-8000 pg/mL的宽检测范围，可满足不同浓度样本的检测需求，实验操作流程标准化，适用于体外细胞因子分泌分析、感染或炎症模型中免疫应答评估、疫苗研发过程中的免疫效果监测等科研场景。试剂盒包含预包被捕获抗体的96孔板、检测抗体及配套标准品，支持科研人员高效开展猪源样本中IL-8的定量检测，为动物免疫学研究提供可靠工具。
别名：

CXCL8 ELISA Kit; IL8Interleukin-8 ELISA Kit; IL-8 ELISA Kit; Alveolar macrophage chemotactic factor I ELISA Kit; AMCF-I ELISA Kit; C-X-C motif chemokine 8 ELISA Kit; Chemokine ELISA Kit; C-X-C motif) ligand 8 ELISA Kit
缩写：

CXCL8
Uniprot No.：

P26894
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

125 pg/mL-8000 pg/mL
灵敏度：

31.25 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	91-99
1:2	Average %	90
	Range %	86-94
1:4	Average %	88
	Range %	84-93
1:8	Average %	92
	Range %	88-97

回收率：

The recovery of pig IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	95-104
EDTA plasma (n=4)	85	81-90

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
8000	2.662	2.601	2.632	2.460
4000	2.215	2.161	2.188	2.016
2000	1.498	1.404	1.451	1.279
1000	0.871	0.819	0.845	0.673
500	0.510	0.507	0.509	0.337
250	0.380	0.359	0.370	0.198
125	0.271	0.261	0.266	0.094
0	0.174	0.170	0.172

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Taurine ameliorates deoxynivalenol-induced intestinal injury in piglets: Restoration of mitochondrial function linked to the PGC1α-NRF1/2 axis X Ji, H Ding, F Zhou, F Zhang, D Wu,Ecotoxicology and Environmental Safety,2025
Allicin Improves Intestinal Epithelial Barrier Function and Prevents LPS-Induced Barrier Damages Jingxia Gao,Frontiers in immunology,2022
Porcine circovirus type 2 upregulates endothelial-derived IL-8 production in porcine iliac artery endothelial cells via the RIG-I/MDA-5/MAVS/JNK signaling pathway F Shi,BMC Veterinary Research,2020
Dietary resveratrol improves antioxidant status of sows and piglets and regulates antioxidant gene expression in placenta by Keap1-Nrf2 pathway and Sirt1 Qingwei Meng.et al,J Anim Sci Biotechnol.,2018
The Effect of Divergence in Feed Efficiency on the Intestinal Microbiota and the Intestinal Immune Response in Both Unchallenged and Lipopolysaccharide Challenged Ileal and Colonic Explants. Vigors S. et al,PLoS One.,2016

平均分:

5.0分 - 1 个评价

样品类型：细胞培养上清液

样品信息：其他

稀释比：没有稀释

产品评价：结构很好，已发文章

By 高老师

最新研究进展：

IL8，也称为白细胞介素-8，是一种细胞因子蛋白质，参与炎症和免疫反应。它主要由激活的单核细胞和巨噬细胞产生，在炎症、感染和损伤等条件下被释放。IL8作为趋化因子，可以引导白细胞移动到感染或受损组织，促进炎症反应和免疫细胞的增殖和激活。最近的研究表明，IL8也参与许多其他生物学过程，如细胞增殖、生存、转移和血管新生等。
功能：

IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus.
基因功能参考文献：
1. This study evaluated the subcellular localization and other functions of TGEV nsp7 protein through analysis of its effects on cell growth, cell cycle progression, interleukin 8 (IL-8) expression, and NF-kappaB activation. PMID: 29521102
2. The JNK-activated AP-1 subunit c-Jun was critical for the up-regulation of IL-8 expression by PRRSV PMID: 28347884
3. These results suggest that porcine circovirus 2 induces IL-8 secretion via the TLR2/MyD88/NF-kappaB signalling pathway. PMID: 26581603
4. Facilitated degradation of the RGS16 by porcine circovirus type 2 ORF3 further enhances NFkappaB translocation into the nucleus through the ERK1/2 signalling pathway and increased IL-6 and IL-8 mRNA transcripts. PMID: 25575706
5. Suggest that the interplay between TGFbeta-2 and LPS regulates the levels of IL-8 in the immature newborn intestine. PMID: 25147235
6. downregulated gene expression elite controllers (fraction of human population displaying resistance to HIV infection) PMID: 23751822
7. Data suggest that MSK1 and MSK2 are the major CREB kinases in fibroblast-like synoviocytes from rheumatoid arthritis patients stimulated with lysophosphatidic acid and that phosphorylation of CREB1 at Ser-133 plays a significant role in IL-8 production. PMID: 24792438
8. Study presents evidence demonstrating a single species of exotoxin ApxI, derived from A. pleuropneumoniae serotype 10, induces the expression and production of proinflammatory cytokines IL-1beta, IL-8 and TNF-alpha in porcine alveolar macrophages. PMID: 21314908
9. local expression of IL-1beta and IL-8 in non-bacterial thrombotic endocarditis, Staphylococcus aureus infective endocarditis, animals with S. aureus sepsis without endocarditis and controls PMID: 23879680
10. In swine, IL-8, TNF-ALPHA, INOS AND MIP-1BETA were increased during mechanical ventilation in a time-related fashion. PMID: 23102097
11. Classical swine fever virus NS2 protein promotes interleukin-8 expression. PMID: 21318239
12. Developmental changes in IL-8 isoforms may serve to minimize its inflammatory effects in the fetus and also provide a mechanism to restore its full activity after birth PMID: 19232500
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亚细胞定位：

Secreted.
蛋白家族：

Intercrine alpha (chemokine CxC) family
组织特异性：

Alveolar macrophages.
数据库链接：

KEGG: ssc:396880

STRING: 9823.ENSSSCP00000026976

UniGene: Ssc.658

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.