Pig interleukin 2,IL-2 ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO猪白介素2（IL-2）酶联免疫检测试剂盒（货号：CSB-E06783p），采用双抗体夹心法定量检测血清、血浆及组织匀浆样本中的IL-2蛋白浓度。IL-2作为T淋巴细胞分泌的核心细胞因子，在调节免疫应答、促进淋巴细胞增殖分化中发挥关键作用，是研究猪类免疫调控机制的重要分子指标。试剂盒检测灵敏度为15.6 pg/mL，线性范围覆盖15.6-1000 pg/mL，实验全程约需4.5小时完成，包含预包被特异性抗体的酶标板及标准品、检测抗体、显色液等完整组分。该试剂盒适用于科研领域中对猪类免疫系统功能的评估，例如疫苗研发中免疫激活效果分析、传染病模型中细胞免疫反应监测，或通过检测组织内IL-2表达水平探究炎症性疾病的分子机制。实验操作全程无需特殊设备，常规酶标仪在450nm波长下即可完成读数，为畜牧兽医研究、比较免疫学及动物疾病模型构建提供可靠工具。
别名：

IL2Interleukin-2 ELISA Kit; IL-2 ELISA Kit; T-cell growth factor ELISA Kit; TCGF ELISA Kit
缩写：

IL2
Uniprot No.：

P26891
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	80-99
1:2	Average %	86
	Range %	82-91
1:4	Average %	91
	Range %	86-97
1:8	Average %	94
	Range %	88-98

回收率：

The recovery of pig IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-102
EDTA plasma (n=4)	96	91-102

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
基因功能参考文献：
1. We observed that besides TCR stimulation Tregs require IL-2 for activation. PMID: 21530576
2. a parameter of early mRNA expression during intestinal ischemia reperfusion injury PMID: 15050130
3. The isolation and characterization of interleukin 2 from Tibetan swine are reported. PMID: 16195934
亚细胞定位：

Secreted.
蛋白家族：

IL-2 family
数据库链接：

KEGG: ssc:396868

STRING: 9823.ENSSSCP00000009697

UniGene: Ssc.16224