Pig hepatocyte growth factor,HGF ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

猪肝细胞生长因子(HGF)酶联免疫试剂盒（CSB-E06795p）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的HGF含量。HGF（肝细胞生长因子）是一种多功能细胞因子，在胚胎发育、组织再生与修复等生理过程中发挥关键作用。其异常表达与肿瘤生长、侵袭和转移密切相关。研究机制主要是通过与受体c-Met结合，激活下游信号通路，影响细胞的增殖、存活等。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，可广泛应用于肝脏再生机制研究、疾病模型（如肝纤维化或损伤修复）中HGF动态变化的检测，以及药物或生物制剂干预后HGF调控效应的评估，为相关基础研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

HGF
Uniprot No.：

F1SB93
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

62.5 pg/mL-4000 pg/mL
灵敏度：

15.6 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cancer
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig HGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	84-92
1:2	Average %	104
	Range %	94-110
1:4	Average %	95
	Range %	89-99
1:8	Average %	94
	Range %	86-97

回收率：

The recovery of pig HGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	90-95
EDTA plasma (n=4)	97	92-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.