Pig glial fibrillary acidic protein,GFAP ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO猪源神经胶质纤维酸性蛋白（GFAP）酶联免疫检测试剂盒（货号CSB-E14950p），采用竞争法原理实现定量分析，可检测血清、血浆及组织匀浆样本中的GFAP含量，检测灵敏度覆盖0.1-20 ng/mL的宽动态范围。GFAP是神经胶质细胞（如星形胶质细胞）的标志性中间丝蛋白，其表达水平与神经系统损伤、炎症或病变状态密切相关，常用于评估中枢神经系统损伤程度及胶质细胞活化情况。本试剂盒通过特异性抗体与样本中GFAP的竞争结合反应实现精准检测，适用于猪模型在神经退行性疾病研究（如阿尔茨海默病、帕金森病）、脑创伤模型评估、神经毒性实验或神经炎症机制探索等科研场景中，定量分析血液或组织样本的GFAP动态变化，为神经病理学研究提供可靠工具。
缩写：

GFAP
Uniprot No.：

F1RR02
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.1 ng/mL-20 ng/mL
灵敏度：

0.05 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Neuroscience
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig GFAP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	84-97
1:2	Average %	96
	Range %	92-99
1:4	Average %	92
	Range %	89-96
1:8	Average %	96
	Range %	90-102

回收率：

The recovery of pig GFAP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	94-105
EDTA plasma (n=4)	96	92-103

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.