Pig fibroblast growth factor 19(FGF19)ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

猪成纤维细胞生长因子19(FGF19)酶联免疫试剂盒（CSB-E17583p）为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的FGF19含量。FGF19是一种成纤维细胞生长因子。它在调节胆汁酸合成、糖脂代谢等方面有重要作用。研究机制上，FGF19结合并激活FGFR4-βKlotho复合物，启动下游信号通路，调控基因表达。相关研究为代谢性疾病等治疗提供潜在靶点。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于猪模型中代谢调控机制研究、肝脏疾病模型构建、营养干预实验等科研场景，可为探究FGF19在能量平衡、肠道功能及病理生理过程中的作用提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

FGF19
Uniprot No.：

F1RY79
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig FGF19 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	80-94
1:2	Average %	99
	Range %	91-107
1:4	Average %	96
	Range %	92-105
1:8	Average %	96
	Range %	88-98

回收率：

The recovery of pig FGF19 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	89-99
EDTA plasma (n=4)	94	90-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.