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Pig endothelin 1,ET-1 ELISA Kit

  • 中文名称:
    猪内皮素1(ET-1)酶联免疫试剂盒
  • 货号:
    CSB-E06806p
  • 规格:
    96T/48T
  • 价格:
    ¥4200/¥3000
  • 其他:

产品详情

  • 产品描述:
    猪内皮素1(ET-1)酶联免疫试剂盒(CSB-E06806p)基于双抗体夹心法原理设计,适用于定量检测猪血清、血浆及组织匀浆样本中的ET-1水平。ET-1是由血管内皮细胞分泌的强效血管收缩肽,参与血压调节、细胞增殖及心血管疾病发生等生理病理过程,是心血管研究领域的重要靶标。本试剂盒检测灵敏度为3.12 pg/mL,线性范围为3.12-200 pg/mL,可在4小时内完成检测,配套提供预包被板、标准品、检测抗体及显色试剂,支持多样本类型分析且无需复杂前处理。该产品适用于科研场景中评估猪心血管疾病模型、药物干预效果或组织微环境中ET-1的动态变化,其宽检测范围和高灵敏度可精准捕捉低丰度样本的浓度差异,为心血管机制研究及药理实验提供可靠工具。
  • 别名:
    EDN1 ELISA Kit; Endothelin-1 ELISA Kit; ET-1 ELISA Kit; Preproendothelin-1 ELISA Kit; PPET1) [Cleaved into: Big endothelin-1] ELISA Kit
  • 缩写:
  • Uniprot No.:
  • 种属:
    Sus scrofa (Pig)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    3.12 pg/mL-200pg/mL
  • 灵敏度:
    0.78 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Cancer
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of pig ET-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %100
    Range %94-105
    1:2Average %97
    Range %92-101
    1:4Average %89
    Range %85-93
    1:8Average %95
    Range %91-101
  • 回收率:
    The recovery of pig ET-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9085-94
    EDTA plasma (n=4)8480-89
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    2002.401 2.449 2.425 2.354
    1001.689 1.661 1.675 1.604
    500.925 0.912 0.919 0.848
    250.491 0.459 0.475 0.404
    12.50.302 0.286 0.294 0.223
    6.250.221 0.213 0.217 0.146
    3.120.158 0.154 0.156 0.085
    00.068 0.074 0.071  
  • 数据处理:
  • 货期:
    3-5 working days

产品评价

靶点详情

  • 功能:
    Endothelins are endothelium-derived vasoconstrictor peptides. Probable ligand for G-protein coupled receptors EDNRA and EDNRB which activates PTK2B, BCAR1, BCAR3 and, GTPases RAP1 and RHOA cascade in glomerular mesangial cells.
  • 基因功能参考文献:
    1. In conclusion, endothelial vWF knockdown prevented angiotensin II-induced ET-1 upregulation through attenuation of NOX-mediated O2- production. PMID: 27443965
    2. sub-vasomotor concentration of ET-1 leads to vascular dysfunction by impairing endothelium-dependent NO-mediated dilation via p38 kinase-mediated production of superoxide from NADPH oxidase following ETA receptor activation PMID: 26211713
    3. ET1 was lowest in kidneys removed from live pigs, greater in kidneys from pigs with brain death, and greatest in kidneys from pigs with cardiac arrest. PMID: 25380860
    4. ET-1 contributes to formation of oedema during experimental sepsis by a novel mechanism involving increased HBP release from neutrophils. PMID: 24611481
    5. Endothelin-1 expression is upregulated following therapeutic hypothermia after cardiac arrest. PMID: 23717659
    6. Our experiment with isolated porcine ophthalmic ciliary arteries revealed a refractoriness phase to ET after an acute stimulation with ET. This refractoriness was transient and disappeared after 4 h. PMID: 23327561
    7. ET-1 produces contraction of the urinary bladder neck muscles via muscular ET(A) receptors coupled to extracellular Ca(2+) entry via VOC (L-type) and non-VOC channels. PMID: 21953705
    8. urinary biomarker to determine kidney graft injury PMID: 22221604
    9. Endothelin-1 elevation is not only a conserved phenomenon in a pig traumatic brain injury (TBI) model, but it is a likely target for understanding the observed enhanced vascular response to TBI. PMID: 21801588
    10. interleukin-6, endothelin ET-1, and apoptotic Bak and Bcl-XL genes have roles in small bowel transplantation, in a swine model of ischemia and reperfusion injury PMID: 21884347
    11. ET-1 elicits a different pattern of Src family kinase (SFK) activation and might trigger a different pattern of SFK activation from that caused by ATP and UTP. PMID: 21792912
    12. Compared with the untreated group, the levels of serum ET-1 after acute myocardial infarction and reperfusion were significantly decreased in the Xuefu Zhuyu-treated group. PMID: 18405606
    13. study suggests a possible role for endothelin-1(resulting from the actions of endothelin-converting enzyme-1) acting via endothelin receptor A in the control of luteolytic sensitivity in the pig PMID: 19783117
    14. vasoconstrictor influence of endogenous as well as exogenous ET on coronary circulation in vivo is reduced in myocardial infarct PMID: 15458948
    15. Data show the presence of a continuative and differentially regulated stimulating mechanism of ET-1 expression during pig endotoxaemia as well as a fundamental role of ET-1 system in the cardiovascular and respiratory derangement. PMID: 16043243
    16. These data support a linkage between the proinflammatory cytokine IL-1beta and vascular dysfunction within the intestinal circulation, mediated, at least in part, by the ET system. PMID: 16782700
    17. extracellular ET-1 regulates the abundance of ET-1 mRNA in PAECs, in an ETB receptor-dependent manner, by modulating both mRNA stability and transcription via mechanisms involving receptor endocytosis and both ERK and p38 MAPK pathways PMID: 18278064
    18. Cyclosporine-A stimulates angiotensin I/II secretion by kidney glomeruli. PMID: 18331442
    19. Left ventricular unloading with intra-aortic counter pulsation prior to reperfusion reduces myocardial release of endothelin-1 and decreases infarction size in a porcine ischemia-reperfusion model. PMID: 18814226
    20. The beneficial effect of carvedilol on myocardial no-reflow could be due to its effect on ET-1 via the activation of the K(ATP) channel. PMID: 18840580
    21. is not predictive of ventricular fibrillation during acute myocardial ischemia PMID: 18844577
    22. Porcine ET-1 rapidly activates the prosurvival phosphatidylinositol 3'-OH kinase (PI3K)/AKT signaling pathway in normal and fibrotic human lung fibroblasts. PMID: 19188658
    23. Report novel pathway involving endothelin-1 and reactive oxygen species in coronary vasospasm with endothelial dysfunction. PMID: 19623039

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  • 亚细胞定位:
    Secreted.
  • 蛋白家族:
    Endothelin/sarafotoxin family
  • 数据库链接: