Pig coagulation factor VIII(FVIII) ELISA Kit

Pig coagulation factor VIII(FVIII) ELISA Kit

产品详情

产品评价

靶点详情

产品详情

产品评价

相关产品

靶点详情

Most popular with customers

产品中心

信号通路

技术信息

联系信息

中文名称：

猪凝血因子VIII(FVIII)酶联免疫试剂盒
货号：

CSB-E15000p
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情
产品评价
相关产品
靶点详情

产品描述：

猪凝血因子VIII（FVIII）作为内源性凝血途径的关键蛋白，在凝血级联反应中发挥核心作用，其活性水平与凝血功能密切相关。CUSABIO试剂盒采用竞争性酶联免疫吸附法（ELISA），定量检测猪血清、血浆或组织匀浆样本中的FVIII含量，检测灵敏度范围为62.5-4000 ng/mL，通过预包被特异性抗体的微孔板捕获样本中的FVIII抗原，与酶标记抗原竞争结合，显色强度与目标物浓度呈负相关。试剂盒包含标准品、检测抗体、显色底物等完整组分，适用于出血性疾病机制研究、凝血调节药物开发、动物模型凝血功能评估等科研领域。实验全程可在3.5小时内完成，支持多样本类型检测，为研究猪FVIII在生理或病理状态下的表达变化提供可靠工具，特别适用于畜牧科学、兽医学研究及生物制药领域的凝血相关实验分析。
别名：

F8 ELISA kit; CF8Coagulation factor VIII ELISA kit; Procoagulant component ELISA kit
缩写：

F8
Uniprot No.：

P12263
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

62.5 ng/mL-4000 ng/mL
灵敏度：

15.62 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig FVIII in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:100	Average %	89
	Range %	84-95
1:200	Average %	94
	Range %	89-99
1:400	Average %	93
	Range %	86-100
1:800	Average %	98
	Range %	91-103

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

4000

0.134

0.128

0.131

2000

0.221

0.237

0.229

1000

0.384

0.398

0.391

500

0.615

0.629

0.622

250

0.868

0.881

0.875

125

1.214

1.253

1.234

62.5

1.649

1.684

1.667

2.236

2.125

2.181

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

Factor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa.
基因功能参考文献：
1. It was concluded that VEGF and factor VIII are important growth factors associated with fetal development in pigs and are identified in all uterine segments. PMID: 28237345
2. Report Factor VIII organisation on nanodiscs with different lipid composition. PMID: 25589466
3. thrombin stimulates transglutaminase activity in articular cartilage by directly cleaving factor XIII and by receptor-mediated up-regulation of factor XIII synthesis PMID: 14983233
4. cupredoxin-like A1 subdomains in fVIII contain inter-species differences that are a result of selective pressure on the dissociation rate constant PMID: 16513639
亚细胞定位：

Secreted, extracellular space.
蛋白家族：

Multicopper oxidase family
数据库链接：

KEGG: ssc:397339

STRING: 9823.ENSSSCP00000028556

UniGene: Ssc.16043

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.