Pig cholecystokinin,CCK ELISA Kit

Pig cholecystokinin,CCK ELISA Kit

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中文名称：

猪胆囊收缩素/肠促胰酶肽(CCK)酶联免疫试剂盒
货号：

CSB-E10131p
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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CUSABIO猪胆囊收缩素/肠促胰酶肽（CCK）酶联免疫检测试剂盒（货号：CSB-E10131p），采用夹心法原理实现定量检测，适用于血清、血浆及组织匀浆样本中CCK的精准分析。胆囊收缩素是一种广泛分布于胃肠道和中枢神经系统的多肽类激素，在调节消化酶分泌、胆囊收缩及食欲调控中发挥关键作用，是研究消化生理和神经内分泌调控的重要靶点。试剂盒检测范围为1.56-100 pg/mL，具有高灵敏度和宽线性范围，可满足低浓度样本检测需求。该产品基于双抗体夹心法开发，通过特异性抗体实现目标蛋白的高效捕获与检测，操作流程标准化，适用于科研领域中对猪源性样本CCK表达水平的定量研究，例如动物消化系统功能评估、神经肽作用机制探索或代谢调控相关实验。通过兼容血清、血浆及多种组织样本的检测特性，本试剂盒为研究者提供灵活的实验方案选择，助力于猪模型在胃肠生理、神经生物学及代谢疾病机制等方向的科研工作。
别名：

CCK ELISA Kit; Cholecystokinin ELISA Kit; CCK) [Cleaved into: Cholecystokinin-58 ELISA Kit; CCK58); Cholecystokinin-58 desnonopeptide ELISA Kit; (1-49)-CCK58); Cholecystokinin-39 ELISA Kit; CCK39); Cholecystokinin-33 ELISA Kit; CCK33); Cholecystokinin-25 ELISA Kit; CCK25); Cholecystokinin-18 ELISA Kit; CCK18); Cholecystokinin-12 ELISA Kit; CCK12); Cholecystokinin-8 ELISA Kit; CCK8); Cholecystokinin-7 ELISA Kit; CCK7); Cholecystokinin-5 ELISA Kit; CCK5)] ELISA Kit
缩写：

CCK
Uniprot No.：

P01356
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

1.56 pg/mL-100 pg/mL
灵敏度：

0.39 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig CCK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	93-100
1:2	Average %	104
	Range %	101-107
1:4	Average %	91
	Range %	85-97
1:8	Average %	90
	Range %	87-93

回收率：

The recovery of pig CCK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	81-89
EDTA plasma (n=4)	92	87-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
100	2.179	2.198	2.189	2.031
50	1.698	1.677	1.688	1.530
25	1.136	1.166	1.151	0.993
12.5	0.697	0.658	0.678	0.520
6.25	0.486	0.497	0.492	0.334
3.12	0.383	0.398	0.391	0.233
1.56	0.301	0.321	0.311	0.153
0	0.157	0.159	0.158

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Dietary Branched-Chain Amino Acids Regulate Food Intake Partly through Intestinal and Hypothalamic Amino Acid Receptors in Piglets Tian M,journal of agricultural and food chemistry,2019
Branched chain amino acids stimulate gut satiety hormone cholecystokinin secretion through the activation of the umami taste receptor T1R1/T1R3 using an in vitro porcine jejunum model Min Tian,Food & Function,2019

功能：

This peptide hormone induces gall bladder contraction and the release of pancreatic enzymes in the gut. Its function in the brain is not clear. Binding to CCK-A receptors stimulates amylase release from the pancreas, binding to CCK-B receptors stimulates gastric acid secretion.
基因功能参考文献：
1. We conclude that only a small fraction of neuronal CCK is nonsulfated. The intracellular distribution of nonsulfated CCK in neurons suggests that they contribute only modestly to the CCK transmitter activity. PMID: 27535680
2. Cardiac expression of pro-cholecystokinin is cell-specific, which differentiates the expression from that of intestinal endocrine cells and cerebral neurons. PMID: 25627687
3. This review explores the role of luminal cholecystokinin on the regulation of exocrine pancreatic secretion in author's studies of neonatal pigs. PMID: 15075451
亚细胞定位：

Secreted.
蛋白家族：

Gastrin/cholecystokinin family
组织特异性：

Synthesized in both cerebral cortex and duodenal mucosa.
数据库链接：

KEGG: ssc:397468

STRING: 9823.ENSSSCP00000012023

UniGene: Ssc.717

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.