Your Good Partner in Biology Research

Pig cardiac troponin Ⅰ,cTn-ⅠELISA Kit

  • 中文名称:
    猪心肌肌钙蛋白Ⅰ(cTn-Ⅰ)酶联免疫试剂盒
  • 货号:
    CSB-E11187p
  • 规格:
    96T/48T
  • 价格:
    ¥4200/¥3000
  • 其他:

产品详情

  • 产品描述:
    猪心肌肌钙蛋白Ⅰ(cTn-Ⅰ)酶联免疫试剂盒(CSB-E11187p)为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的cTn-I含量。cTn-I(心肌肌钙蛋白I)是一种心肌细胞特有的调节蛋白,在心肌损伤时释放进入血液,成为诊断心肌损伤的重要标志物。其研究机制涉及心肌损伤时cTn-I的释放和检测,对于早期诊断心肌梗死等心血管疾病具有重要意义。试剂盒检测范围为15.6 pg/mL-1000 pg/mL,适用于科研领域中心肌损伤相关机制研究;适用于动物模型中心脏疾病研究、心血管药物开发中的药效评估,以及体外实验中心肌细胞损伤模型的生物标志物检测,为科研人员提供稳定可靠的心肌损伤动态监测工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 缩写:
    cTnⅠ
  • Uniprot No.:
  • 种属:
    Sus scrofa (Pig)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    15.6 pg/mL-1000 pg/mL
  • 灵敏度:
    3.9 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Cardiovascular
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of pig cTn-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 94  
    Range % 89-102  
    1:2 Average % 89  
    Range % 85-99  
    1:4 Average % 95  
    Range % 90-100  
    1:8 Average % 93  
    Range % 83-99  
  • 回收率:
    The recovery of pig cTn-Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 97 90-101  
    EDTA plasma (n=4) 95 86-106  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    1000 1.815 1.866 1.841 1.703  
    500 1.652 1.713 1.683 1.545  
    250 1.347 1.401 1.374 1.236  
    125 1.006 0.957 0.982 0.844  
    62.5 0.743 0.725 0.734 0.596  
    31.25 0.514 0.508 0.511 0.373  
    15.6 0.324 0.308 0.316 0.178  
    0 0.136 0.139 0.138    
  • 数据处理:
  • 货期:
    3-5 working days

产品评价