Pig apolipoprotein E (Apo-E) ELISA Kit

中文名称：

猪载脂蛋白E(Apo-E)酶联免疫试剂盒
货号：

CSB-E17889p
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情
产品评价
相关产品
靶点详情

产品详情

产品描述：

CUSABIO猪载脂蛋白E（Apo-E）酶联免疫检测试剂盒（货号CSB-E17889p），采用竞争法定量原理，可精准检测血清、血浆及组织匀浆样本中的Apo-E蛋白浓度。Apo-E作为脂质代谢关键蛋白，在胆固醇运输和脂蛋白代谢调控中发挥重要作用，其水平变化与脂代谢异常等研究密切相关。试剂盒检测范围为31.25-2000 ng/mL，可覆盖猪相关研究中常见的生物样本浓度区间，实验操作采用预包被微孔板与生物素标记抗体系统，配备即用型标准品和显色底物，支持96孔板高通量检测。试剂盒包含冻干标准品（需-20℃保存）、检测抗体（-20℃保存）及预包被板（4℃保存）等组件，在规范保存条件下有效期达6个月。本产品适用于科研领域中对猪脂代谢机制、营养干预模型或相关病理生理研究的Apo-E水平分析，为动物实验样本提供稳定、特异的检测方案，实验全程优化操作步骤，支持3小时内完成检测流程。
别名：

APOEApolipoprotein E ELISA kit; Apo-E ELISA kit
缩写：

APOE
Uniprot No.：

P18650
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

31.25 ng/mL-2000 ng/mL
灵敏度：

7.81 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig Apo-E in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	96
	Range %	85-105
1:200	Average %	90
	Range %	89-98
1:400	Average %	96
	Range %	82-103
1:800	Average %	97
	Range %	92-99

回收率：

The recovery of pig Apo-E spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-105
EDTA plasma (n=4)	95	85-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
2000	0.131	0.134	0.133
1000	0.223	0.226	0.225
500	0.335	0.341	0.338
250	0.562	0.553	0.558
125	0.862	0.860	0.861
62.5	1.192	1.214	1.203
31.25	1.589	1.607	1.598
0	2.417	2.395	2.406

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

产品评价

靶点详情

功能：

APOE is an apolipoprotein, a protein associating with lipid particles, that mainly functions in lipoprotein-mediated lipid transport between organs via the plasma and interstitial fluids. APOE is a core component of plasma lipoproteins and is involved in their production, conversion and clearance. Apoliproteins are amphipathic molecules that interact both with lipids of the lipoprotein particle core and the aqueous environment of the plasma. As such, APOE associates with chylomicrons, chylomicron remnants, very low density lipoproteins (VLDL) and intermediate density lipoproteins (IDL) but shows a preferential binding to high-density lipoproteins (HDL). It also binds a wide range of cellular receptors including the LDL receptor/LDLR and the very low-density lipoprotein receptor/VLDLR that mediate the cellular uptake of the APOE-containing lipoprotein particles. Finally, APOE has also a heparin-binding activity and binds heparan-sulfate proteoglycans on the surface of cells, a property that supports the capture and the receptor-mediated uptake of APOE-containing lipoproteins by cells.
基因功能参考文献：
1. These data suggest that the -155T>A mutation in the promoter region of the porcine APOE gene is an important functional variant PMID: 21549015
亚细胞定位：

Secreted. Secreted, extracellular space. Secreted, extracellular space, extracellular matrix.
蛋白家族：

Apolipoprotein A1/A4/E family
数据库链接：

KEGG: ssc:397576

STRING: 9823.ENSSSCP00000003343

UniGene: Ssc.1342

Pig apolipoprotein E (Apo-E) ELISA Kit

产品详情

产品评价

相关产品

靶点详情

Most popular with customers

产品中心

信号通路

技术信息

联系信息