Pig apolipoprotein A1 (Apo-A1) ELISA Kit

Pig apolipoprotein A1 (Apo-A1) ELISA Kit

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中文名称：

猪载脂蛋白A1(Apo-A1)酶联免疫试剂盒
货号：

CSB-E06834p
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

猪载脂蛋白A1（Apo-A1）酶联免疫试剂盒（货号：CSB-E06834p）是一款基于竞争法原理设计的定量检测试剂盒，适用于检测猪血清、血浆或组织匀浆样本中的Apo-A1浓度。Apo-A1是高密度脂蛋白（HDL）的主要载脂蛋白成分，参与胆固醇逆向转运过程，在脂质代谢调控和心血管健康中发挥关键作用。本试剂盒检测范围为80-2000 μg/mL，通过预先包被特异性抗体的微孔板捕获样本中的Apo-A1，在竞争反应体系中，样本内源性Apo-A1与标记物竞争结合抗体，最终通过酶标系统显色，其信号强度与目标物浓度呈负相关。该试剂盒具有高灵敏度和特异性，能够稳定检测不同基质样本中的Apo-A1水平，适用于猪脂代谢机制研究、心血管疾病动物模型评估、营养干预对脂蛋白代谢的影响分析等科研领域。实验操作全程可在常规实验室条件下完成，配备标准品和优化缓冲体系，保障检测结果的稳定性和重复性，为猪相关代谢性疾病的基础研究提供可靠工具。
别名：

APOA1Apolipoprotein A-I ELISA Kit; Apo-AI ELISA Kit; ApoA-I ELISA Kit; Apolipoprotein A1) [Cleaved into: Proapolipoprotein A-I ELISA Kit; ProapoA-I); Truncated apolipoprotein A-I] ELISA Kit
缩写：

APOA1
Uniprot No.：

P18648
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

80 μg/mL-2000 μg/mL
灵敏度：

50 μg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig Apo-A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	89-103
1:2	Average %	90
	Range %	85-103
1:4	Average %	93
	Range %	88-100
1:8	Average %	89
	Range %	82-97

回收率：

The recovery of pig Apo-A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	96-104
EDTA plasma (n=4)	90	86-95

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

μg/ml	OD1	OD2	Average
2000	0.342	0.348	0.345
800	0.533	0.483	0.508
480	0.682	0.657	0.670
200	1.021	0.987	1.004
80	1.267	1.325	1.296
0	1.903	1.954	1.929

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
基因功能参考文献：
1. Alterations in the Apo A-I pattern is a good indicator of the presence and severity of infectious diseases in the pig.Lower overall amounts of Apo A-I were observed in Salmonella typhimurium and Escherichia coli infections. PMID: 25377528
2. blood level decreases during experimentally induced acute-phase processes in pigs PMID: 15845530
3. down-regulation of apolipoprotein A-I and A-IV messages in the liver may be mediated by interleukin 6 and tumor necrosis factor-alpha PMID: 15878672
亚细胞定位：

Secreted.
蛋白家族：

Apolipoprotein A1/A4/E family
组织特异性：

Major protein of plasma HDL, also found in chylomicrons. Synthesized predominantly in the intestine and the liver.
数据库链接：

KEGG: ssc:397691

STRING: 9823.ENSSSCP00000015994

UniGene: Ssc.807

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.