Pig anti-Mullerian hormone (AMH) ELISA kit

Pig anti-Mullerian hormone (AMH) ELISA kit

产品详情

引用文献

产品评价

靶点详情

产品详情

引用文献

产品评价

相关产品

靶点详情

Most popular with customers

产品中心

信号通路

技术信息

联系信息

中文名称：

猪缪勒管抑制物质/抗缪勒管激素(AMH)酶联免疫试剂盒
货号：

CSB-E13408p
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情
产品评价
引用文献
相关产品
靶点详情

产品描述：

本试剂盒为猪缪勒管抑制物质/抗缪勒管激素（AMH）的定量检测提供高灵敏度解决方案，采用竞争性酶联免疫吸附法（ELISA）原理，通过预包被特异性抗体实现精准分析。AMH作为转化生长因子β超家族成员，在哺乳动物生殖调控中发挥重要作用，尤其参与卵泡发育及性别分化过程。该试剂盒可检测血清、血浆及组织匀浆样本，线性检测范围为1.25-50 ng/mL，灵敏度达0.31 ng/mL，实验全程仅需3小时完成，配套提供标准品、检测抗体、显色底物等完整组分，试剂盒组分需于2-8℃保存并在有效期内使用。适用于动物生殖生理研究、繁殖效率评估、卵巢功能分析等科研领域，特别为猪科动物生殖内分泌研究提供可靠工具。其检测机制基于样本中的AMH与酶标记抗原竞争结合抗体位点，最终显色强度与AMH浓度呈负相关，通过标准曲线实现准确定量，为科研人员探索AMH在生殖调控中的作用机制提供高效检测平台。
别名：

AMH ELISA Kit; Muellerian-inhibiting factor ELISA Kit; Anti-Muellerian hormone ELISA Kit; AMH ELISA Kit; Muellerian-inhibiting substance ELISA Kit; MIS ELISA Kit
缩写：

AMH
Uniprot No.：

P79295
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

1.25 ng/mL-50 ng/mL
灵敏度：

1.25 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig AMH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	89-102
1:2	Average %	93
	Range %	90-103
1:4	Average %	90
	Range %	85-100
1:8	Average %	93
	Range %	83-107

回收率：

The recovery of pig AMH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-103
EDTA plasma (n=4)	97	88-107

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
50	0.134	0.139	0.137
25	0.227	0.238	0.233
11.5	0.330	0.343	0.337
3.75	0.611	0.592	0.602
1.25	1.264	1.214	1.239
0	2.686	2.635	2.661

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Early indicators of a gill's reproductive potential LM Staveley,International journal of STD & AIDS,2024

功能：

This glycoprotein, produced by the Sertoli cells of the testis, causes regression of the Muellerian duct. It is also able to inhibit the growth of tumors derived from tissues of Muellerian duct origin.
亚细胞定位：

Secreted.
蛋白家族：

TGF-beta family
数据库链接：

UniGene: Ssc.97577

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.