Pig Vascular cell adhesion molecule 1,VCAM-1 ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO猪血管内皮细胞粘附分子1（VCAM-1/CD106）酶联免疫检测试剂盒（货号CSB-E06825p），采用双抗体夹心法原理实现定量检测，适用于科研领域分析血清、血浆及组织匀浆样本中的VCAM-1含量。VCAM-1是表达于血管内皮细胞表面的免疫球蛋白超家族成员，通过介导白细胞与内皮细胞的粘附作用参与炎症反应和免疫调节，其表达水平变化与血管炎症、动脉粥样硬化等病理过程密切相关。试剂盒检测灵敏度达15.6ng/mL，线性检测范围为15.6-1000ng/mL，配备预包被捕获抗体和生物素标记检测抗体，通过标准品梯度浓度建立定量曲线，可精准测定样本中VCAM-1的浓度。该试剂盒适用于研究猪心血管疾病模型中的内皮细胞活化机制、炎症性疾病病理进程评估，以及免疫调节药物对血管内皮功能影响的体外实验，为探索VCAM-1在血管相关疾病中的作用提供可靠工具，实验全程需配合酶标仪在450nm波长下完成读数分析。
缩写：

VCAM1/CD106
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

15.6ng/mL-1000ng/mL
灵敏度：

3.9 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig VCAM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	85-94
1:2	Average %	94
	Range %	87-98
1:4	Average %	103
	Range %	97-107
1:8	Average %	101
	Range %	95-104

回收率：

The recovery of pig VCAM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	89	83-94

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.