Pig Syndecan-1/CD138(SDC1) ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO猪Syndecan-1/CD138（SDC1）酶联免疫试剂盒（货号：CSB-E17413p），采用双抗体夹心法定量检测血清、血浆及组织匀浆样本中SDC1的蛋白水平。SDC1是一种跨膜蛋白聚糖，在细胞黏附、信号转导及组织微环境调控中发挥重要作用，其表达水平变化常与炎症反应、组织修复等生理病理过程相关。试剂盒检测灵敏度为5 ng/mL，线性范围为5-320 ng/mL，标准曲线覆盖低、中、高浓度梯度，适用于动态监测样本中靶蛋白含量变化。试剂盒包含预包被捕获抗体的96孔板、系列浓度标准品、HRP标记检测抗体及显色底物，操作流程包含样本孵育、洗涤、显色和终止步骤，实验全程约需4.5小时。未开封试剂盒建议4℃保存，开封后组分需-20℃分装保存以避免反复冻融。该产品可应用于猪模型研究中SDC1蛋白的表达分析，如评估炎症性疾病进程、组织再生机制研究或作为特定病理状态的潜在生物标志物探索，仅限科研用途。
缩写：

SDC1
Uniprot No.：

K7GNF5
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

5 ng/mL-320 ng/mL
灵敏度：

1.25 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig SDC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-90
1:2	Average %	93
	Range %	87-102
1:4	Average %	97
	Range %	92-102
1:8	Average %	91
	Range %	80-98

回收率：

The recovery of pig SDC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	81-98
EDTA plasma (n=4)	98	90-105

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.