Pig Soluble protein-100B,S-100B ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO猪S100B蛋白（S-100B）酶联免疫检测试剂盒（货号：CSB-E14063p），采用双抗体夹心法原理实现精确定量分析，适用于猪血清、血浆或组织匀浆样本中S100B蛋白含量的检测，灵敏度范围为78 pg/mL至5000 pg/mL。S100B蛋白属于钙结合蛋白家族，主要表达于神经胶质细胞和雪旺细胞中，参与调控细胞周期、能量代谢及细胞内钙稳态，其表达水平变化与神经损伤、炎症反应及中枢神经系统疾病密切相关。本试剂盒通过高特异性抗体对目标蛋白进行捕获与检测，可稳定应用于神经损伤模型研究、疾病机制探索或药物干预效果评价等科研场景，为分析猪体内S100B蛋白的动态变化提供可靠工具。样本兼容性强，支持直接检测未经稀释的体液样本或经适当处理的组织匀浆，操作流程标准化，满足实验室对神经生物学、兽医学或比较医学等领域的研究需求。
缩写：

S-100B
Uniprot No.：

I3L5R6
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

78 pg/mL-5000 pg/mL
灵敏度：

19.5 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig S-100B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	91-100
1:2	Average %	93
	Range %	85-101
1:4	Average %	86
	Range %	80-98
1:8	Average %	94
	Range %	92-105

回收率：

The recovery of pig S-100B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	83-102
EDTA plasma (n=4)	95	92-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.