Pig Progonadoliberin-2(GNRH2) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

猪促性腺素释放素原-2(GNRH2)酶联免疫试剂盒（CSB-EL009636PI）为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GNRH2含量。GNRH2即促性腺激素释放激素2，它在生殖内分泌系统中扮演重要角色。研究认为它通过与特异性受体结合，调控垂体促性腺激素的合成与分泌，进而影响生殖功能。目前对其作用机制的深入研究，有望为生殖疾病的诊治提供新方向。试剂盒检测范围为40 pg/mL-8000 pg/mL，广泛应用于动物生殖生理机制研究，包括繁殖性能评估、生殖内分泌调控网络解析以及激素相关疾病模型的分子机制探索，也可为畜牧业中繁殖管理策略的优化提供数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

GnRH-II, LH-RHII, OTTHUMP00000030076|OTTHUMP00000030077|luliberin II|luteinizing hormone-releasing hormone II|progonadoliberin II
缩写：

GNRH2
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

40 pg/mL-8000 pg/mL
灵敏度：

20 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig GNRH2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	81-92
1:2	Average %	96
	Range %	90-105
1:4	Average %	97
	Range %	92-105
1:8	Average %	94
	Range %	88-103

回收率：

The recovery of pig GNRH2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-99
EDTA plasma (n=4)	97	89-105

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.