Pig Peptide YY(PYY) ELISA kit

Pig Peptide YY(PYY) ELISA kit

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中文名称：

猪多肽YY(PYY)酶联免疫试剂盒
货号：

CSB-EL019128PI
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO猪多肽YY（PYY）酶联免疫试剂盒（货号CSB-EL019128PI），采用竞争法原理实现定量检测，适用于分析血清、血浆样本中的PYY含量，检测范围为62.5-1000 pg/mL。PYY是一种由肠道L细胞分泌的神经内分泌激素，通过作用于中枢神经系统参与食欲调控和能量代谢平衡，其表达水平的变化与胃肠道功能、营养吸收等生理过程密切相关。本试剂盒基于抗原-抗体的特异性结合反应设计，通过预包被靶标蛋白捕获样本中的游离PYY，结合酶标二抗显色后，浓度与吸光度呈负相关性，可实现精准定量分析。该产品适用于科研领域探索猪的消化生理机制、营养干预研究或代谢相关疾病模型的构建，例如肥胖、糖尿病等动物实验中的激素动态监测，为解析PYY在能量稳态中的作用提供可靠工具。试剂盒操作便捷，标准曲线覆盖广，重复性良好，可满足实验室对微量样本的高通量检测需求，适用于基础医学、兽医学及动物营养学等领域的科学研究。
别名：

PYY ELISA Kit; Peptide YY ELISA Kit; PYY ELISA Kit; Peptide tyrosine tyrosine) [Cleaved into: Peptide YY(3-36 ELISA Kit; PYY-II)] ELISA Kit
缩写：

PYY
Uniprot No.：

P68005
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma
检测范围：

62.5 pg/mL-1000 pg/mL
灵敏度：

16.88 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Neuroscience
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig PYY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	87-99
1:2	Average %	96
	Range %	91-100
1:4	Average %	94
	Range %	89-98
1:8	Average %	93
	Range %	87-99

回收率：

The recovery of pig PYY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	91-101
EDTA plasma (n=4)	94	89-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
1000	0.171	0.175	0.173
500	0.352	0.353	0.353
250	0.681	0.676	0.679
125	1.269	1.272	1.271
62.5	2.858	2.867	2.863

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Sodium Butyrate Protects the Intestinal Barrier by Modulating Intestinal Host Defense Peptide Expression and Gut Microbiota After a Challenge With Deoxynivalenol in … Wang S,J Agric Food Chem,2020
Effects of Dietary Fiber Sources during Gestation on Stress Status, Abnormal Behaviors and Reproductive Performance of Sows Huang S,Animals (Basel),2020
Dietary Branched-Chain Amino Acids Regulate Food Intake Partly through Intestinal and Hypothalamic Amino Acid Receptors in Piglets Tian M,journal of agricultural and food chemistry,2019
Potential Link between Gut Microbiota and Deoxynivalenol-Induced Feed Refusal in Weaned Piglets Wang S, et al,journal of agricultural and food chemistry,2019

功能：

This gut peptide inhibits exocrine pancreatic secretion, has a vasoconstrictory action and inhibitis jejunal and colonic mobility.
亚细胞定位：

Secreted.
蛋白家族：

NPY family
数据库链接：

STRING: 9823.ENSSSCP00000023751

UniGene: Ssc.53428

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.