Pig Nuclear factor-kappa B,NF-κB ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

猪核因子-κB(NF-κB)酶联免疫试剂盒（CSB-E13448p）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的NF-κB含量。NF - κB是重要的转录因子，广泛存在于多种细胞中。在细胞的存活、增殖、炎症反应等生理病理过程中起到关键作用。研究机制上，它受多种信号通路调控，激活后能转位至细胞核调控基因表达，其异常与肿瘤、炎症等多种疾病相关。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，该产品适用于动物模型构建、病原体感染机制研究、疫苗效力评估等科研领域本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

NF-kappa B,Nuclear factor-kappa B
缩写：

NF-κB
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.81 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Epigenetics and Nuclear Signaling
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig NF-κB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	87-95
1:2	Average %	103
	Range %	99-106
1:4	Average %	93
	Range %	87-98
1:8	Average %	91
	Range %	88-95

回收率：

The recovery of pig NF-κB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	92-102
EDTA plasma (n=4)	90	86-93

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.