Pig Neuron-specific enolase,NSE ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO试剂盒采用双抗体夹心酶联免疫法，专为定量检测猪血清、血浆或组织匀浆中的神经特异性烯醇化酶（NSE）而设计。NSE作为糖酵解关键酶，在神经内分泌细胞和神经元中特异性高表达，其水平变化常与神经损伤、中枢神经系统疾病及神经内分泌肿瘤等病理过程相关，是神经生物学研究的重要分子标记物。试剂盒检测灵敏度达0.195 ng/mL，线性范围覆盖0.78-50 ng/mL，采用预包被特异性抗体的96孔板，通过生物素化检测抗体与HRP标记链霉亲和素系统实现精准检测，显色强度与样本中NSE浓度呈正相关。适用于脑组织、外周神经等样本的神经功能评估，兼容常规血清/血浆样本（需离心去除沉淀后分装冻存），可满足神经退行性疾病模型构建、神经损伤修复研究以及神经内分泌相关疾病机制探索等科研需求，为动物实验中的病理机制解析和药物干预效果观察提供可靠定量工具。
缩写：

NSE
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.78 ng/mL-50 ng/mL
灵敏度：

0.195 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cancer
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig NSE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-102
1:2	Average %	90
	Range %	85-99
1:4	Average %	95
	Range %	87-98
1:8	Average %	94
	Range %	86-104

回收率：

The recovery of pig NSE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	84-102
EDTA plasma (n=4)	94	83-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.