Pig Myeloperoxidase (MPO) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO猪髓过氧化物酶（MPO）酶联免疫检测试剂盒（货号CSB-E09397p），采用双抗体夹心法原理实现定量分析，适用于检测猪血清、血浆及组织匀浆样本中MPO蛋白含量。MPO是中性粒细胞颗粒中关键的过氧化物酶，通过催化次氯酸生成参与先天免疫防御，其表达水平与炎症反应、氧化应激及组织损伤密切相关。试剂盒检测灵敏度为15.6 ng/mL，线性范围覆盖15.6-1000 ng/mL，通过预包被特异性捕获抗体与标记检测抗体的协同作用，可精准量化目标蛋白浓度。该试剂盒适用于科研领域中对感染性疾病模型、免疫调控机制、心血管或神经退行性疾病的病理研究，也可用于评估抗炎药物或抗氧化剂对MPO活性的干预效果。实验操作兼容常规酶标仪分析，提供标准品、检测抗体及显色系统等完整组分，满足实验室对生物样本中MPO蛋白表达水平的稳定检测需求。
缩写：

MPO
Uniprot No.：

K7GRV6
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

15.6 ng/mL-1000 ng/mL
灵敏度：

3.9 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig MPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	91
	Range %	87-99
1:10	Average %	97
	Range %	93-105
1:20	Average %	85
	Range %	82-89
1:40	Average %	90
	Range %	85-98

回收率：

The recovery of pig MPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-95
EDTA plasma (n=4)	95	89-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.