Pig Lysyl Oxidase ELISA Kit

中文名称：

猪赖氨酰氧化酶酶联免疫试剂盒
货号：

CSB-E15813p
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

猪赖氨酰氧化酶酶联免疫试剂盒（CSB-E15813p）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LOX含量。LOX（脂氧化酶）是一种催化花生四烯酸生成生物活性分子的关键酶，广泛分布于人体，参与细胞信号传导和代谢。研究发现，LOX在多种恶性肿瘤中表达增高，其代谢产物与肿瘤的发生、发展密切相关。研究LOX的分子生物学特征和促癌机制，有助于开发新的抗肿瘤药物。试剂盒检测范围为1.56 ng/mL-100 ng/mL，可广泛应用于猪模型的组织病理研究，例如评估纤维化疾病进程中LOX的表达动态、肿瘤转移相关基质硬化研究，或心血管疾病模型中胶原沉积的分子机制解析，为畜牧疾病模型构建和基础医学研究提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

LOX ELISA Kit; Protein-lysine 6-oxidase ELISA Kit; EC 1.4.3.13 ELISA Kit; Lysyl oxidase) [Cleaved into: Protein-lysine 6-oxidase ELISA Kit; long form; Protein-lysine 6-oxidase ELISA Kit; short form] ELISA Kit
缩写：

LOX
Uniprot No.：

P45845
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

1.56 ng/mL-100 ng/mL
灵敏度：

0.39 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig lysyl oxidase in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	81-89
1:2	Average %	93
	Range %	90-95
1:4	Average %	106
	Range %	101-111
1:8	Average %	98
	Range %	94-102

回收率：

The recovery of pig lysyl oxidase spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	83	80-85
EDTA plasma (n=4)	106	103-109

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
100	2.073	2.000	2.037	1.950
50	1.616	1.636	1.626	1.539
25	1.149	1.201	1.175	1.088
12.5	0.721	0.735	0.728	0.641
6.25	0.440	0.457	0.449	0.362
3.12	0.286	0.269	0.278	0.191
1.56	0.198	0.187	0.193	0.106
0	0.085	0.089	0.087

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

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功能：

Responsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. Regulator of Ras expression. May play a role in tumor suppression. Plays a role in the aortic wall architecture.
亚细胞定位：

Secreted. Secreted, extracellular space.
蛋白家族：

Lysyl oxidase family
数据库链接：

STRING: 9823.ENSSSCP00000015138

UniGene: Ssc.10386

Pig Lysyl Oxidase ELISA Kit

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