Pig Interleukin-23 subunit alpha(IL23A) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO猪白介素-23 α亚基（IL23A）酶联免疫检测试剂盒（货号：CSB-EL011638PI），采用双抗体夹心法定量检测猪血清、血浆或组织匀浆样本中IL23A的蛋白水平。IL23A是IL-23细胞因子的功能亚基，在调节Th17细胞分化及介导炎症反应中发挥重要作用，其表达水平与免疫调节和疾病进程密切相关。试剂盒通过高特异性抗体对实现精准检测，线性检测范围为31.25 pg/mL至2000 pg/mL，可灵敏识别样本中微量IL23A的浓度变化。实验流程包含预包被抗体、样本孵育、生物素标记抗体反应及显色分析等标准化步骤，适用于科研领域对猪免疫系统功能的研究，如感染性疾病模型建立、免疫调节机制解析或炎症相关分子通路的探索。试剂盒提供完整的标准品、检测缓冲液及操作说明书，支持实验室高效开展IL23A蛋白水平的定量分析，为动物免疫学研究提供标准化工具。
别名：

IL23A; SGRF; Interleukin-23 subunit alpha; IL-23 subunit alpha; IL-23-A; Interleukin-23 subunit p19; IL-23p19
缩写：

IL23A
Uniprot No.：

Q9N2H9
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.8 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig IL23A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-95
1:2	Average %	102
	Range %	97-107
1:4	Average %	91
	Range %	85-97
1:8	Average %	97
	Range %	91-103

回收率：

The recovery of pig IL23A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	94	90-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Associates with IL12B to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
基因功能参考文献：
1. These results provide genetic information about both swine IL-23 the IL-23 receptor alpha (IL-23Ralpha) and IL-12 receptor beta1 (IL-12Rbeta1), which allows for better understanding of interleukin systems involved in pig immunity. PMID: 22032895
亚细胞定位：

Secreted.
蛋白家族：

IL-6 superfamily
数据库链接：

KEGG: ssc:100155546

UniGene: Ssc.56047