Pig Interleukin 22(IL-22) ELISA Kit

产品详情

产品描述：

CUSABIO猪白介素22（IL-22）定量检测试剂盒（货号CSB-E15847p），采用双抗体夹心法原理，专为科研领域设计，可精准检测血清、血浆及组织匀浆样本中的IL-22蛋白水平。IL-22作为重要的细胞因子，在黏膜免疫应答、组织修复及炎症调控中发挥关键作用，对猪类免疫机制研究和疾病模型构建具有重要价值。试剂盒检测范围为78 pg/mL至5000 pg/mL，具备高灵敏度与宽线性范围，可覆盖多数实验场景需求。操作流程包含标准品梯度稀释、样本孵育、酶标抗体结合及显色反应，配备预包被捕获抗体、生物素标记检测抗体及HRP-链霉亲和素放大系统，显色底物经终止反应后可通过酶标仪读取450 nm吸光度值完成定量分析。适用于动物免疫调控研究、病原感染后细胞因子表达分析、肠道屏障功能评估等科研方向，为探索猪类疾病模型、疫苗效果评价及营养干预研究提供可靠工具。试剂盒含完整实验组分及操作指南，支持96孔板规格，保存条件为2-8℃，保质期12个月。
缩写：

IL-22
Uniprot No.：

F1SH68
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

78 pg/mL-5000 pg/mL
灵敏度：

19.5 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-22 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	99
	Range %	94-104
1:2	Average %	96
	Range %	89-100
1:4	Average %	95
	Range %	89-98
1:8	Average %	103
	Range %	95-107

回收率：

The recovery of pig IL-22 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	91-103
EDTA plasma (n=4)	101	95-107

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.