Pig Interleukin 1α,IL-1α ELISA kit

Pig Interleukin 1α,IL-1α ELISA kit

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中文名称：

猪白介素1α(IL-1α)酶联免疫试剂盒
货号：

CSB-E09725p
规格：

96T/48T
价格：

￥3800/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

本产品是专为科研设计的猪白介素1α(IL-1α)定量检测试剂盒（货号CSB-E09725p），采用双抗体夹心法原理，可精准检测血清、血浆、细胞培养上清液及组织匀浆中的IL-1α含量。IL-1α作为促炎性细胞因子，在炎症反应、免疫调节及组织修复中起关键作用，其水平变化与感染性疾病模型、免疫应激研究及动物实验中的病理机制密切相关。试剂盒检测灵敏度高，线性范围覆盖25-1600 pg/mL，通过预包被特异性捕获抗体与酶标检测抗体的协同作用，实现对目标蛋白的特异性识别，适用于复杂生物样本的稳定检测。该试剂盒可为猪类动物模型中的炎症机制研究、疫苗开发评估、疾病进程监测等科研项目提供可靠数据支持，例如在猪繁殖与呼吸综合征、猪流行性腹泻等感染性疾病模型中定量分析IL-1α的动态变化，或在免疫调控实验中评估细胞因子网络的作用机制。操作流程严格遵循标准ELISA技术规范，配备完整实验组分，确保科研数据的可重复性和准确性。
别名：

IL1A ELISA Kit; Interleukin-1 alpha ELISA Kit; IL-1 alpha ELISA Kit
缩写：

IL1A
Uniprot No.：

P18430
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

25 pg/mL-1600 pg/mL
灵敏度：

6.25 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-93
1:2	Average %	104
	Range %	100-108
1:4	Average %	91
	Range %	85-97
1:8	Average %	87
	Range %	83-92

回收率：

The recovery of pig IL-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	95-103
EDTA plasma (n=4)	95	90-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1600	2.599	2.535	2.567	2.385
800	1.839	1.899	1.869	1.687
400	1.174	1.197	1.186	1.004
200	0.753	0.742	0.748	0.566
100	0.460	0.499	0.480	0.298
50	0.368	0.372	0.370	0.188
25	0.284	0.292	0.288	0.106
0	0.181	0.183	0.182

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Altered Cytokine Expression and Barrier Properties after In Vitro Infection of Porcine Epithelial Cells with Enterotoxigenic Escherichia coli and Probiotic Enterococcus faecium Martina Kern.et al,Mediators of Inflammation,2017

功能：

Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
基因功能参考文献：
1. Constitutive suppression of IL-1alpha maintains quiescence of endothelium and that terminal complement complexes remove that suppression, allowing IL-1alpha transcription and, ultimately, activation of endothelium to proceed. PMID: 18292602
亚细胞定位：

Cytoplasm. Secreted.
蛋白家族：

IL-1 family
数据库链接：

KEGG: ssc:397094

STRING: 9823.ENSSSCP00000008638

UniGene: Ssc.113

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.