Pig Interleukin 18,IL-18 ELISA Kit

Pig Interleukin 18,IL-18 ELISA Kit

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中文名称：

猪白介素18(IL-18)酶联免疫试剂盒
货号：

CSB-E06781p
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO猪白介素18（IL-18）酶联免疫检测试剂盒（货号：CSB-E06781p），采用双抗体夹心法定量检测猪血清、血浆或组织匀浆样本中的IL-18蛋白浓度。IL-18是一种重要的促炎性细胞因子，广泛参与天然免疫和适应性免疫的调控，在感染性疾病、炎症反应及免疫病理研究中具有关键作用。试剂盒检测范围为31.25-2000 pg/mL，可精准覆盖猪体内IL-18的生理及病理浓度变化，最低检测限达31.25 pg/mL，适用于科研场景中动态监测实验动物模型（如细菌/病毒感染、免疫调节研究）的免疫应答水平。该试剂盒提供预包被特异性抗体的96孔板，配套标准品、检测抗体及显色系统，操作流程标准化，可在4.5小时内完成检测。样本兼容性广泛，支持血清/血浆直接检测及组织样本的匀浆预处理，为兽医学、比较免疫学及动物疾病模型研究提供可靠工具，助力科研人员解析IL-18在猪免疫系统中的分子机制与调控网络。
别名：

IL18 ELISA Kit; IGIF ELISA Kit; Interleukin-18 ELISA Kit; IL-18 ELISA Kit; Interferon gamma-inducing factor ELISA Kit; IFN-gamma-inducing factor ELISA Kit; Interleukin-1 gamma ELISA Kit; IL-1 gamma ELISA Kit
缩写：

IL18
Uniprot No.：

O19073
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.81 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	82-92
1:2	Average %	100
	Range %	95-105
1:4	Average %	91
	Range %	85-97
1:8	Average %	97
	Range %	91-103

回收率：

The recovery of pig IL-18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	84-93
EDTA plasma (n=4)	94	91-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.562	2.687	2.625	2.443
1000	1.839	1.865	1.852	1.670
500	1.104	1.135	1.120	0.938
250	0.643	0.638	0.641	0.459
125	0.412	0.435	0.424	0.242
62.5	0.321	0.341	0.331	0.149
31.25	0.262	0.248	0.255	0.073
0	0.181	0.183	0.182

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Interaction between Porcine Alveolar Macrophage-Tang Cells and Streptococcus suis Strains of Different Virulence: Phagocytosis and Apoptosis S Li,Microorganisms,2023
Multi-omics unravel the compromised mucosal barrier function linked to aberrant mucin O-glycans in a pig model B Xia,International journal of biological macromolecules,2023
Effects of resveratrol and its derivative pterostilbene on hepatic injury and immunological stress of weaned piglets challenged with lipopolysaccharide Y Li,Journal of Animal Science,2022
Mucin O-glycan-microbiota axis orchestrates gut homeostasis in a diarrheal pig model B Xia,Microbiome,2022

功能：

A proinflammatory cytokine primarily involved in polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses. Upon binding to IL18R1 and IL18RAP, forms a signaling ternary complex which activates NF-kappa-B, triggering synthesis of inflammatory mediators. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells and natural killer (NK) cells.
基因功能参考文献：
1. Study reports that the monocyte-derived cytokines IL-12 and IL-18 act synergistically on porcine gammadelta T cells to induce IFN-gamma production. Additional stimuli such as the mitogen ConA and IL-2 are necessary to activate the cells for enhanced proliferation. PMID: 25036760
2. IL-18 concentration in saliva was significantly increased during a 60-min acute immobilization stress in thirteen 5-month-old pigs. These results are the first evidence of a stress-related change of IL-18 in pig saliva. PMID: 21682650
3. endometrial expression of CASP1 and IL18 associated with pregnancy establishment; alteration of CASP1 and IL18 following premature exposure of uterus to estrogen during early pregnancy may contribute to conceptus loss between Days 15 to 18 of pregnancy PMID: 20380728
4. Porcine IL-18 regulates anti-pig cellular rejection in C57BL/6 mice. PMID: 17079874
亚细胞定位：

Cytoplasm. Secreted.
蛋白家族：

IL-1 family
数据库链接：

KEGG: ssc:397057

STRING: 9823.ENSSSCP00000015962

UniGene: Ssc.20

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.