Pig Interleukin 15(IL-15) ELISA Kit

Pig Interleukin 15(IL-15) ELISA Kit

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中文名称：

猪白介素15(IL-15)酶联免疫试剂盒
货号：

CSB-E14320p
规格：

96T/48T
价格：

￥3800/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

猪白介素15（IL-15）酶联免疫试剂盒（货号：CSB-E14320p）是一款基于双抗体夹心法原理设计的定量检测试剂盒，适用于检测猪血清、血浆、细胞培养上清液、组织匀浆及细胞裂解液等多种样本中的IL-15蛋白水平。IL-15是一种重要的免疫调节细胞因子，在先天性和适应性免疫应答中发挥关键作用，尤其在NK细胞和记忆性T细胞的增殖与活化过程中具有重要功能。本试剂盒检测灵敏度高，线性范围为0.78 pg/mL至50 pg/mL，可精准测定样本中极低浓度的IL-15含量。其检测体系通过预包被特异性捕获抗体与标记检测抗体的协同作用，确保检测结果的特异性和稳定性。该产品广泛应用于动物免疫学、疾病机制研究及疫苗开发等领域，例如用于评估猪群免疫状态、研究病原体感染过程中细胞因子的动态变化，或分析体外免疫细胞培养模型中IL-15的分泌水平，为科研人员探索猪类免疫调控网络提供可靠工具。
别名：

IL15 ELISA Kit; Interleukin-15 ELISA Kit; IL-15 ELISA Kit
缩写：

IL15
Uniprot No.：

Q95253
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates, cell lysates
检测范围：

0.78 pg/mL-50 pg/mL
灵敏度：

0.195 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-15 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-89
1:2	Average %	102
	Range %	97-107
1:4	Average %	87
	Range %	84-91
1:8	Average %	84
	Range %	81-89

回收率：

The recovery of pig IL-15 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	97-103
EDTA plasma (n=4)	84	80-88

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
50	2.602	2.576	2.589	2.400
25	1.879	1.850	1.865	1.676
12.5	1.144	1.168	1.156	0.967
6.25	0.683	0.691	0.687	0.498
3.12	0.452	0.468	0.460	0.271
1.56	0.361	0.391	0.376	0.187
0.78	0.288	0.298	0.293	0.104
0	0.191	0.186	0.189

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

β-hydroxy-β-methylbutyrate modulates lipid metabolism in adipose tissues of growing pigs Yehui Duan.et al,FOOD & FUNCTION,2018
Branched-chain amino acid ratios modulate lipid metabolism in adipose tissues of growing pigs Yehui Duan.et al,Journal of Functional Foods,2018

功能：

Cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL15 requires interaction of IL15 with components of the IL2 receptor, including IL2RB and probably IL2RG but not IL2RA. In neutrophils, stimulates phagocytosis probably by signaling through the IL15 receptor, composed of the subunits IL15RA, IL2RB and IL2RG, which results in kinase SYK activation.
基因功能参考文献：
1. Study identified two completely linked SNPs in the porcine IL15 promoter region that could alter IL15 transcription activity. As interleukin-15 can inhibit porcine adipocyte differentiation, these promoter mutations could affect intramuscular fat deposition by producing differential levels of muscle-derived interleukin-15. PMID: 29168191
2. Myokine IL-15 regulates the crosstalk of co-cultured porcine skeletal muscle satellite cells and preadipocytes. PMID: 25098601
3. results demonstrate that porcine reproductive and respiratory syndrome virus (PRRSV)infection could induce IL-15 production in macrophages/dendritic cells; data further show that upregulation of IL-15 by PRRSV requires PKC and NF-kappaB pathways PMID: 22573868
4. IFN-gamma targets the adipocyte to induce IL-15 expression, thus indicating a possible role for the adipocyte in the regulation of T-cell function and muscle metabolism during the innate immune response PMID: 14656772
5. When induced by IFN-gamma or other inflammatory mediators, IL-15 may be a significant homeorhetic factor that mobilizes and directs energy away from the adipocyte to other cells during the acute phase of the inflammatory response. PMID: 15155279
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亚细胞定位：

Secreted.
蛋白家族：

IL-15/IL-21 family
数据库链接：

KEGG: ssc:397683

STRING: 9823.ENSSSCP00000009657

UniGene: Ssc.8833

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.