Pig Interleukin 12,IL-12/P40 ELISA KIT

Pig Interleukin 12,IL-12/P40 ELISA KIT

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中文名称：

猪白介素12(IL-12/P40)酶联免疫试剂盒
货号：

CSB-E11341p
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO定量检测猪白介素12(IL-12/P40)的酶联免疫试剂盒，采用双抗体夹心法原理，适用于血清、血浆及细胞培养上清液样本的精准分析。IL-12/P40作为促炎性细胞因子的重要亚基，在免疫调节和Th1细胞分化中发挥关键作用，其水平检测可为感染性疾病、自身免疫研究及疫苗开发等科研领域提供重要参考。试剂盒检测灵敏度达15.6 pg/mL，线性范围覆盖15.6-1000 pg/mL，实验过程包含预包被特异性捕获抗体的酶标板、生物素标记检测抗体及链霉亲和素-HRP系统，通过标准曲线实现目标蛋白的定量分析。该试剂盒适用于评估实验动物模型中的免疫应答强度、细胞因子网络调控机制研究，或体外药物筛选过程中炎症介质的动态监测。操作流程兼容常规实验室设备，配套提供标准品、检测缓冲液及显色底物，确保实验的可重复性和稳定性，为科研人员探索猪源IL-12/P40在免疫病理学中的功能提供可靠工具。
别名：

IL12BInterleukin-12 subunit beta ELISA Kit; IL-12B ELISA Kit; Cytotoxic lymphocyte maturation factor 40 kDa subunit ELISA Kit; CLMF p40 ELISA Kit; IL-12 subunit p40 ELISA Kit
缩写：

IL12B
Uniprot No.：

Q28938
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, cell culture supernates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-12/P40 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	80-99
1:2	Average %	95
	Range %	90-104
1:4	Average %	98
	Range %	92-110
1:8	Average %	95
	Range %	85-105

回收率：

The recovery of pig IL-12/P40 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	85-104
EDTA plasma (n=4)	98	90-112

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.028	2.157	2.093	1.884
500	1.562	1.612	1.587	1.378
250	0.980	1.034	1.007	0.798
125	0.570	0.603	0.587	0.378
62.5	0.352	0.362	0.357	0.148
31.25	0.303	0.311	0.307	0.098
15.6	0.282	0.268	0.275	0.066
0	0.214	0.204	0.209

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.; Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
基因功能参考文献：
1. Data show that cysteine significantly reduced the expression of pro-inflammatory cytokines, including TNF-alpha, IL-6, IL-12p40, IL-1beta, and resulted in increased expression of the apoptosis initiator caspase-8 and bcl2L1. PMID: 19520150
亚细胞定位：

Secreted.
蛋白家族：

Type I cytokine receptor family, Type 3 subfamily
数据库链接：

KEGG: ssc:397076

STRING: 9823.ENSSSCP00000018057

UniGene: Ssc.71

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.