Pig Interleukin 1 receptor antagonist, IL-1ra ELISA kit

产品详情

产品描述：

猪白介素1受体拮抗剂(IL1Ra)酶联免疫试剂盒（CSB-E04946p）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL1RN含量。IL1RN是重要的靶点。它是白细胞介素-1受体拮抗剂，可与IL-1竞争性结合IL-1受体，抑制IL-1的促炎作用。在研究中，聚焦其在炎症性疾病如类风湿关节炎等发病机制中的作用，以探索新的治疗策略。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，该产品广泛应用于科研领域，包括但不限于猪类炎症性疾病模型的机制研究、免疫调节药物开发中的靶标验证，以及感染或应激条件下宿主免疫反应的动态监测，为探索IL - 1信号通路在动物生理病理过程中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

IL1RN ELISA kit; IRAP1Interleukin-1 receptor antagonist protein ELISA kit; IL-1RN ELISA kit; IL-1ra ELISA kit; IRAP ELISA kit; IL1 inhibitor ELISA kit
缩写：

IL1RN
Uniprot No.：

Q29056
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

62.5 pg/mL-4000 pg/mL
灵敏度：

15.6 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-1ra in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	106
	Range %	93-110
1:2	Average %	101
	Range %	91-105
1:4	Average %	97
	Range %	87-101
1:8	Average %	91
	Range %	84-95

回收率：

The recovery of pig IL-1ra spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	85-96
EDTA plasma (n=4)	108	98-112

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity.
基因功能参考文献：
1. This study identified IL-1Ra as an early immunomodulatory mediator that could be involved in the immunopathogenesis of porcine reproductive and respiratory syndrome virus infections. PMID: 27902420
亚细胞定位：

Secreted.
蛋白家族：

IL-1 family
数据库链接：

UniGene: Ssc.16250