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  4. Pig Interleukin 1β,IL-1β ELISA Kit

Pig Interleukin 1β,IL-1β ELISA Kit

文献引用 (27) 评价 (1) 说明书下载
  • 中文名称：
    猪白介素1β (IL-1β)酶联免疫试剂盒
  • 货号：
    CSB-E06782p
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO猪白介素1β（IL-1β）酶联免疫检测试剂盒（货号CSB-E06782p），基于双抗体夹心法定量原理开发，适用于血清、血浆、细胞培养上清及组织匀浆样本中IL-1β的精准检测。IL-1β作为关键促炎细胞因子，在宿主免疫应答、炎症调控及感染性疾病研究中具有重要价值，其表达水平与猪类疾病模型构建及免疫机制探索密切相关。试剂盒检测灵敏度达3.9 pg/mL，线性范围覆盖3.9-250 pg/mL，采用高特异性配对抗体实现靶标分子的高效捕获与检测，确保数据的可重复性。该产品可广泛应用于动物医学研究领域，包括但不限于感染性疾病模型的炎症反应评估、免疫调节药物开发中的疗效监测，以及疫苗研发过程中细胞因子动态变化分析，为科研人员提供稳定可靠的检测平台。试剂盒配套标准品及优化缓冲体系，支持多样本类型同步处理，满足实验室高通量研究需求。
  • 别名：
    IL1BInterleukin-1 beta ELISA kit; IL-1 beta ELISA kit
  • 缩写：
    IL1B
  • Uniprot No.：
  • 种属：
    Sus scrofa (Pig)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    3.9 pg/mL-250 pg/mL
  • 灵敏度：
    0.975 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %88
    Range %80-100
    1:2Average %96
    Range %91-103
    1:4Average %95
    Range %90-110
    1:8Average %95
    Range %85-105
  • 回收率：
    The recovery of pig IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9583-104
    EDTA plasma (n=4)9890-108
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    2502.675 2.815 2.745 2.597
    1252.154 2.147 2.151 2.003
    62.51.312 1.335 1.324 1.176
    31.250.788 0.764 0.776 0.628
    15.60.377 0.356 0.367 0.219
    7.80.264 0.269 0.267 0.119
    3.90.190 0.188 0.189 0.041
    00.153 0.143 0.148 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

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产品评价

平均分:
5.0分 - 1 个评价

样品类型：细胞培养上清液

样品信息：其他

稀释比：没有稀释

产品评价： 结果很满意，也是对比了别家的试剂盒，最后发现还是华美的好用，我用的是细胞培养基上清，别家的试剂盒有干扰因素，华美的没有，试了好几家，也就这个牌子的好用。

By 高老师

相关产品

IL1B Proteins

相关问答

■ 常见问题解答
Q:

Hello, please advise if the Pig IL-1b ELISA kit (#CSB-E06782p) recognizes only mature IL-1b or both mature and pro-IL-1b protein. Thank you.

A:
Thanks for your inquiry. This kit detects total IL-1b, including mature form and pro form.
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靶点详情

  • 功能：
    Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6.
  • 基因功能参考文献：
    1. Analysis of host-protein interactions for African swine fever virus L83L identified IL-1beta as its host ligand. A recombinant African swine fever virus lacking the L83L was developed from the highly virulent field isolate Georgia2007 and was used to show that L83L is a non-essential gene. PMID: 29605728
    2. This study showed that the presence of embryos in oviducts on days 2-3 after mating may influence the oviductal expression of the members of the IL-1beta system, determining the action of IL-1beta, which may be considered to be the earliest sign of pregnancy in pigs. PMID: 28532837
    3. This study showed that classical swine fever virus and p7 protein induced IL-1beta secretion and that p7 protein was a short-lived protein degraded by the proteasome. PMID: 25146005
    4. local expression of IL-1beta and IL-8 in non-bacterial thrombotic endocarditis, Staphylococcus aureus infective endocarditis, animals with S. aureus sepsis without endocarditis and controls PMID: 23879680
    5. IL1B regulates expression of IL1R1 and IL1RAP and stimulates expression of PTGS1 and PTGS2 that are considered to be the most rate-limiting enzymes for endometrial synthesis of prostaglandins during the peri-implantation period of pregnancy in pigs. PMID: 22572995
    6. the results presented here strongly suggest IL-1beta as a key molecule guiding tissue remodelling events after myocardial infarction. PMID: 20678474

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  • 亚细胞定位：
    Cytoplasm, cytosol. Lysosome. Secreted, extracellular exosome. Secreted.
  • 蛋白家族：
    IL-1 family
  • 数据库链接：

    KEGG: ssc:397122

    STRING: 9823.ENSSSCP00000008636

    UniGene: Ssc.28829

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