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  4. Pig Interferon γ,IFN-γ ELISA Kit

Pig Interferon γ,IFN-γ ELISA Kit

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  • 中文名称：
    猪γ干扰素(IFN-γ)酶联免疫试剂盒
  • 货号：
    CSB-E06794p
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO猪γ干扰素（IFN-γ）定量检测试剂盒（货号CSB-E06794p），采用双抗体夹心酶联免疫法原理，可精准检测血清、血浆及组织匀浆样本中15.6-1000 pg/mL浓度范围的IFN-γ蛋白。IFN-γ是由活化T细胞和NK细胞分泌的关键细胞因子，在抗病毒免疫、巨噬细胞激活及Th1型免疫应答中发挥核心作用。本试剂盒通过高特异性抗体对实现靶标捕获与信号放大，实验流程包含标准品梯度稀释、样本孵育、显色反应及酶标仪检测步骤，具备灵敏度高（最低检测限15.6 pg/mL）和线性范围广的特点，适用于猪类动物模型中感染性疾病研究、疫苗免疫效果评估、免疫调节机制探索等科研领域，为评估宿主免疫状态及炎症反应提供可靠工具。试剂盒含预包被96孔板、标准品、检测抗体及全套实验试剂，支持用户灵活开展批量样本分析。
  • 别名：
    IFNGInterferon gamma ELISA Kit; IFN-gamma ELISA Kit
  • 缩写：
    IFNG
  • Uniprot No.：
  • 种属：
    Sus scrofa (Pig)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 94  
    Range % 90-99  
    1:2 Average % 92  
    Range % 85-99  
    1:4 Average % 95  
    Range % 91-102  
    1:8 Average % 95  
    Range % 90-100  
  • 回收率：
    The recovery of pig IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 86-102  
    EDTA plasma (n=4) 96 89-100  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    1000 2.692 2.647 2.670 2.581  
    500 2.404 2.465 2.435 2.346  
    250 1.974 1.989 1.982 1.893  
    125 1.523 1.541 1.532 1.443  
    62.5 0.945 0.942 0.944 0.855  
    31.2 0.613 0.635 0.624 0.535  
    15.6 0.337 0.324 0.331 0.242  
    0 0.090 0.087 0.089 　  
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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IFNG Proteins

IFNG ELISA kits

靶点详情

  • 功能：
    Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation. Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription. Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits. In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading. Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference. Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL. Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation.
  • 基因功能参考文献：
    1. The regulatory effect of IFN-gamma on CYP3A29 expression is mediated via PXR. PMID: 25413352
    2. Mapping of QTL for mycoplasma and tetanus antibodies and IFNgamma. PMID: 20567833
    3. Data show taht the expression of PoIFN-gamma in insect cells was confirmed by Western Blot, indirect immunofluorescence assay and indirect ELISA. PMID: 17577980
    4. IFNgamma production from the frozen peripheral blood mononuclear cells was significantly higher than that from the fresh ones. PMID: 19766617
    5. Translational control of IL-18 expression by its 5'-UTR limits production of IL-18, resulting in restricted expression of mRNA and protein IFN-gamma in this model of crescentic glomerulonephritis(GN). Might amplify CD8+-mediated macrophage-dependent GN. PMID: 12787406
    6. selective changes in immature crypt cells induced by IFN-gamma bound to extracellular matrix could contribute to inappropriate responsiveness to commensal bacteria in inflammatory bowel diseases PMID: 15894119
    7. A functional single nucleotide polymorphism is reported in the coding region of IFN-gamma cDNA that markedly reduces antiviral activity of the IFN-gamma protein. PMID: 17416419
    8. The expression of IFN-gamma in recombiannt Lactococcus lactis as a tool for investigating downregulation of allergic predisposition of pigs to experimental food allergy is reported. PMID: 18164876

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Type II (or gamma) interferon family
  • 组织特异性：
    Released primarily from activated T lymphocytes.
  • 数据库链接：

    KEGG: ssc:396991

    STRING: 9823.ENSSSCP00000000511

    UniGene: Ssc.4093

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |