Pig Interferon β,IFN-β/IFNB ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

猪β干扰素（IFN-β/IFNB）是先天免疫应答中重要的细胞因子，具有抗病毒、免疫调节及抑制细胞增殖等功能，在病毒感染模型构建、免疫机制研究和疫苗开发等领域具有重要科研价值。本试剂盒（货号：CSB-E09890p）采用双抗体夹心法ELISA原理，可定量检测血清、血浆、组织匀浆及细胞裂解液样本中的IFN-β蛋白，检测灵敏度为50 pg/mL，线性范围覆盖50-2000 pg/mL，通过预包被高特异性捕获抗体与酶标记检测抗体的协同作用，确保检测结果的稳定性和准确性。该产品适用于体外科研场景中猪IFN-β的动态水平监测，如病毒感染后免疫应答研究、炎症模型评估或生物制剂开发过程中的效价分析，配套提供标准品及优化后的反应缓冲体系，支持多样本类型直接检测。实验全程无需复杂预处理，96孔板设计可满足高通量检测需求，为免疫学、兽医学及生物制药领域的科研工作者提供可靠工具。
缩写：

IFN-β/IFNB
Uniprot No.：

Q29412
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

50 pg/mL-2000 pg/mL
灵敏度：

25 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig IFN-β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	85-86
1:2	Average %	97
	Range %	92-101
1:4	Average %	89
	Range %	84-93
1:8	Average %	95
	Range %	90-99

回收率：

The recovery of pig IFN-β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-101
EDTA plasma (n=4)	84	80-88