Pig Hepcidin(HAMP) ELISA kit

Pig Hepcidin(HAMP) ELISA kit

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中文名称：

猪铁调素(HAMP)酶联免疫试剂盒
货号：

CSB-EL010124PI
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

猪铁调素(HAMP)酶联免疫试剂盒（CSB-EL010124PI）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的HAMP含量。试剂盒检测范围为9.38 ng/mL-600 ng/mL，可为猪铁代谢机制研究、营养性缺铁模型构建、疾病状态下铁调素动态变化分析等科研项目提供可靠工具，尤其适用于农业科学、兽医学领域中对铁稳态调控的分子机制探索及生物标志物筛选。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

HAMP ELISA Kit; Hepcidin ELISA Kit
缩写：

HAMP
Uniprot No.：

Q8MJ80
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

9.38 ng/mL-600 ng/mL
灵敏度：

2.34 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig HAMP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	103
	Range %	99-109
1:2	Average %	94
	Range %	89-99
1:4	Average %	98
	Range %	93-103
1:8	Average %	89
	Range %	84-95

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

Corrected

600

2.751

2.634

2.692

2.543

300

2.164

2.267

2.215

2.066

150

1.424

1.368

1.396

1.247

0.859

0.829

0.844

0.695

37.5

0.428

0.421

0.424

0.275

18.75

0.283

0.273

0.278

0.129

9.38

0.201

0.194

0.198

0.049

0.153

0.145

0.149

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Comparison of Oral and Parenteral Iron Administration on Iron Homeostasis, Oxidative and Immune Status in Anemic Neonatal Pigs Dong Z, et al,Biological Trace Element Research,2019

功能：

Liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of ferroportin/SLC40A1, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma. Controls the major flows of iron into plasma: absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes.; Has strong antimicrobial activity against E.coli ML35P N.cinerea and weaker against S.epidermidis, S.aureus and group b streptococcus bacteria. Active against the fungus C.albicans. No activity against P.aeruginosa.
基因功能参考文献：
1. this study demonstrates that urine hepcidin-25 concentrations strongly correlate with hepatic hepcidin mRNA abundance, plasma hepcidin-25 levels, iron transferrin saturation and non-heme liver iron levels. PMID: 26323096
2. Data suugest that hepcidin might had antiinflammatory function and is a candidate regulator of the cross-talk between iron regulation and inflammation. PMID: 25774043
3. report the full-length cDNA sequences of porcine hepcidin and liver-expressed antimicrobial peptide-2 (LEAP-2) PMID: 16051358
亚细胞定位：

Secreted.
蛋白家族：

Hepcidin family
数据库链接：

KEGG: ssc:397207

STRING: 9823.ENSSSCP00000028422

UniGene: Ssc.376

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.