Pig Glucagon(GC) ELISA Kit

Pig Glucagon(GC) ELISA Kit

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中文名称：

猪胰高血糖素(GC)酶联免疫试剂盒
货号：

CSB-E04854p
规格：

96T/48T
价格：

￥3800/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

猪胰高血糖素（GC）酶联免疫试剂盒（货号：CSB-E04854p）是一款基于竞争法原理开发的定量检测工具，适用于检测猪血清、血浆及组织匀浆样本中的胰高血糖素水平。胰高血糖素是由胰岛α细胞分泌的多肽激素，在调节糖代谢、促进肝糖原分解及维持血糖稳态中发挥关键作用，其水平变化与能量代谢、应激反应等生理过程密切相关。本试剂盒检测范围为125 pg/mL至2000 pg/mL，可精准覆盖猪体内GC的生理及病理浓度区间。实验采用预包被抗体的酶标板，通过样本中GC与标记物的竞争性结合实现定量分析，显色信号强度与目标物浓度呈负相关，操作简便且重复性良好。该产品可广泛应用于动物营养学、代谢性疾病模型研究、激素调控机制探索等科研领域，例如通过检测不同饲喂条件或疾病状态下猪的GC动态变化，为糖代谢研究、药效评价及生理调控提供数据支持。试剂盒配套标准品及优化缓冲体系，确保实验高效稳定，仅限科学研究使用。
别名：

GCGGlucagon [Cleaved into: Glicentin; Glicentin-related polypeptide ELISA Kit; GRPP); Oxyntomodulin ELISA Kit; OXM ELISA Kit; OXY); Glucagon; Glucagon-like peptide 1 ELISA Kit; GLP-1); Glucagon-like peptide 1(7-37 ELISA Kit; GLP-1(7-37)); Glucagon-like peptide 1(7-36 ELISA Kit; GLP-1(7-36)); Glucagon-like peptide 2 ELISA Kit; GLP-2)] ELISA Kit
缩写：

GCG
Uniprot No.：

P01274
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

125 pg/mL-2000 pg/mL
灵敏度：

62.5 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig GC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	85-96
1:2	Average %	95
	Range %	92-102
1:4	Average %	84
	Range %	80-88
1:8	Average %	90
	Range %	85-96

回收率：

The recovery of pig GC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	87-97
EDTA plasma (n=4)	96	90-103

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
2000	0.120	0.111	0.116
1000	0.315	0.340	0.328
500	0.601	0.611	0.606
250	1.018	0.945	0.982
125	2.036	1.923	1.980

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Evaluation of Tannin Extracts, Leonardite and Tributyrin Supplementation on Diarrhoea Incidence and Gut Microbiota of Weaned Piglets M Dell'Anno,Animals,2021

功能：

Plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes.; Potent stimulator of glucose-dependent insulin release. Also stimulates insulin release in response to IL6. Plays important roles on gastric motility and the suppression of plasma glucagon levels. May be involved in the suppression of satiety and stimulation of glucose disposal in peripheral tissues, independent of the actions of insulin. Has growth-promoting activities on intestinal epithelium. May also regulate the hypothalamic pituitary axis (HPA) via effects on LH, TSH, CRH, oxytocin, and vasopressin secretion. Increases islet mass through stimulation of islet neogenesis and pancreatic beta cell proliferation. Inhibits beta cell apoptosis.; Stimulates intestinal growth and up-regulates villus height in the small intestine, concomitant with increased crypt cell proliferation and decreased enterocyte apoptosis. The gastrointestinal tract, from the stomach to the colon is the principal target for GLP-2 action. Plays a key role in nutrient homeostasis, enhancing nutrient assimilation through enhanced gastrointestinal function, as well as increasing nutrient disposal. Stimulates intestinal glucose transport and decreases mucosal permeability.; Significantly reduces food intake. Inhibits gastric emptying in humans. Suppression of gastric emptying may lead to increased gastric distension, which may contribute to satiety by causing a sensation of fullness.; May modulate gastric acid secretion and the gastro-pyloro-duodenal activity. May play an important role in intestinal mucosal growth in the early period of life.
基因功能参考文献：
1. Data suggest that casein, safflower oil, sucrose, and sweetening agent rebaudioside A stimulate GLP1 release/secretion from enteroendocrine cells. (GLP1 = glucagon-like peptide 1, a peptide fragment derived from proglucagon) PMID: 27142747
2. The patterns of colocalization of the K cell marker, glucagon-like insulinotropic peptide, and L cell markers, glucagon like peptide-1 and peptide YY, in enteroendocrine cells of the small intestine and colon of mouse and pig, were investigated. PMID: 25378285
亚细胞定位：

Secreted.; [Glucagon-like peptide 1]: Secreted.
蛋白家族：

Glucagon family
组织特异性：

Glucagon is secreted in the A cells of the islets of Langerhans. GLP-1, GLP-2, oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract. GLP-1 and GLP-2 are also secreted in selected neurons in the brain.
数据库链接：

KEGG: ssc:397595

STRING: 9823.ENSSSCP00000016844

UniGene: Ssc.17225

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.