Pig Follicle-stimulating hormone,FSH ELISA Kit

Pig Follicle-stimulating hormone,FSH ELISA Kit

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中文名称：

猪促卵泡素(FSH)酶联免疫试剂盒
货号：

CSB-E06791p
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO猪促卵泡素（FSH）酶联免疫检测试剂盒（货号：CSB-E06791p），采用双抗体夹心法定量检测血清、血浆及组织匀浆样本中的FSH浓度。FSH是一种由垂体前叶分泌的糖蛋白激素，在生殖调控中发挥关键作用，可促进卵泡发育、雌激素合成以及精子生成，是评估动物繁殖性能的重要指标。试剂盒检测线性范围覆盖20 mIU/mL至700 mIU/mL，适用于多种生物学样本的定量分析，操作流程包含标准品梯度稀释、样本预处理、孵育反应及酶标仪检测等步骤。该试剂盒可广泛应用于畜牧科研领域，如繁殖生理机制研究、生殖障碍模型分析、促排卵药物效果评估以及种猪繁殖力筛选等场景，为动物生殖健康研究提供可靠的数据支持。所有试剂均经过严格质控，板内预包被高特异性抗体，用户仅需按照说明书进行标准化操作即可在4.5小时内完成检测，实验数据稳定可重复。本产品仅限科学研究使用。
别名：

FSHBFollitropin subunit beta ELISA Kit; Follicle-stimulating hormone beta subunit ELISA Kit; FSH-B ELISA Kit; FSH-beta ELISA Kit; Follitropin beta chain ELISA Kit
缩写：

FSH
Uniprot No.：

P01228
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

20 mIU/mL-700 mIU/mL
灵敏度：

10 mIU/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig FSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-94
1:2	Average %	94
	Range %	90-98
1:4	Average %	92
	Range %	87-97
1:8	Average %	84
	Range %	80-88

回收率：

The recovery of pig FSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-99
EDTA plasma (n=4)	97	91-101

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mIU/ml	OD1	OD2	Average	Corrected
700	1.389	1.416	1.403	1.367
250	0.578	0.615	0.597	0.561
100	0.317	0.302	0.310	0.274
50	0.163	0.154	0.159	0.123
20	0.095	0.101	0.098	0.062
0	0.037	0.034	0.036

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Multi-omics analysis reveals gut microbiota-ovary axis contributed to the follicular development difference between Meishan and Landrace× Yorkshire sows B Xu,Journal of animal science and biotechnology,2023
Gut microbiota and blood metabolomics in weaning multiparous sows: Associations with oestrous Xu K,J Anim Physiol Anim Nutr (Berl),2020
Mutated Huntingtin Causes Testicular Pathology in Transgenic Minipig Boars Macakova M.et al,Neurodegener Dis.,2016

功能：

Together with the alpha chain CGA constitutes follitropin, the follicle-stimulating hormone, and provides its biological specificity to the hormone heterodimer. Binds FSHR, a G protein-coupled receptor, on target cells to activate downstream signaling pathways. Follitropin is involved in follicle development and spermatogenesis in reproductive organs.
基因功能参考文献：
1. As in vitro experiments in the present study suggested that LHX2 regulated the expression of Fshbeta, it is possible that LHX2 controls FSH synthesis at the transcriptional level. PMID: 22134063
2. Results showed that the effects of FSHb, ESR, and PRLR genes were significant in the Tibet pig population, and the effective genotypes of the three genes for reproductive traits were BB, BB, and AA, respectively. PMID: 20466637
3. LHX3 LIM homeodomain transcription factor is involved in activation of the FSH beta-subunit gene in the pituitary gonadotrope cell. PMID: 15271874
4. Prop-1 participates in the regulation of FSHbeta gene PMID: 15474519
5. A allele of FSHbeta-1 locus in all the population and FSHbeta-2 locus in pigs of the first parity had the positive effective on TNB, NBA, and WB. PMID: 18065386
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亚细胞定位：

Secreted.
蛋白家族：

Glycoprotein hormones subunit beta family
数据库链接：

KEGG: ssc:396895

STRING: 9823.ENSSSCP00000014160

UniGene: Ssc.5833

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.