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中文名称：猪铁蛋白重链(FTH1)酶联免疫试剂盒
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货号：CSB-EL009030PI
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规格：96T/48T
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价格：￥4200/￥3000
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其他：
产品详情
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产品描述：猪铁蛋白重链(FTH1)酶联免疫试剂盒（CSB-EL009030PI）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FTH1含量。FTH1即铁蛋白重链1，作为重要的铁代谢相关蛋白，在维持细胞内铁稳态中发挥关键作用。其背景与铁离子储存、运输密切相关。研究机制上，它涉及调控铁离子释放，还和氧化应激、肿瘤发生发展等生理病理过程存在关联，是潜在的治疗靶点。试剂盒检测范围为4.7 ng/ml- 300 ng/ml，适用于动物营养学研究中铁代谢动态监测、猪模型铁相关疾病机制探索（如缺铁性贫血或铁过载研究），以及畜牧养殖中饲料铁强化效果评估等科研领域，为体外样本的FTH1定量分析提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
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别名：FTH1 ELISA Kit; FTHFerritin heavy chain ELISA Kit; Ferritin H subunit ELISA Kit; EC 1.16.3.1) [Cleaved into: Ferritin heavy chain ELISA Kit; N-terminally processed] ELISA Kit
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缩写：
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Uniprot No.：
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种属：Sus scrofa (Pig)
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样本类型：serum, plasma, tissue homogenates
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检测范围：4.7 ng/ml- 300 ng/ml
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灵敏度：1.175 ng/ml
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反应时间：1-5h
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样本体积：50-100ul
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检测波长：450 nm
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研究领域：Metabolism
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测定原理：quantitative
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测定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
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线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of pig FTH1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
92
Range %
85-99
1:2
Average %
89
Range %
84-95
1:4
Average %
93
Range %
89-98
1:8
Average %
99
Range %
95-105
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回收率：
The recovery of pig FTH1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
95
88-102
EDTA plasma (n=4)
99
94-103
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标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml
OD1
OD2
Average
Corrected
300
2.734
2.634
2.684
2.525
150
2.183
2.267
2.225
2.066
75
1.678
1.728
1.703
1.544
37.5
1.122
1.129
1.126
0.967
18.75
0.694
0.671
0.682
0.524
9.4
0.453
0.443
0.448
0.289
4.7
0.278
0.284
0.281
0.123
0
0.162
0.155
0.159
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数据处理：
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货期：3-5 working days
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靶点详情
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功能：Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney.; (Microbial infection) Is unable to assume its function upon interaction with viral proteins, thereby increasing Fe concentration in the cytoplasm. This would inhibit the accumulation of reactive oxygen in host cells, leading to reduced apoptosis and increasing the survival of virus infected cell.
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亚细胞定位：Cytoplasm.
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蛋白家族：Ferritin family
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数据库链接：
KEGG: ssc:397030
UniGene: Ssc.23940
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