Pig Erythropoietin,EPO ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO猪红细胞生成素（EPO）酶联免疫检测试剂盒，采用双抗体夹心法定量检测血清、血浆或组织匀浆样本中的EPO浓度。红细胞生成素是由肾脏分泌的重要造血调控激素，在机体缺氧应激和红细胞生成过程中发挥核心作用，其水平变化常作为贫血、肾脏疾病及高原适应等研究的生物标志物。试剂盒灵敏度为1 mIU/mL，检测线性范围覆盖1-40 mIU/mL，可精准测定低丰度样本中EPO的表达量。实验操作采用预包被特异性抗体的96孔板，通过标准品梯度稀释建立定量曲线，配合显色反应实现目标蛋白的浓度测定。该试剂盒适用于动物生理机制研究、疾病模型构建、药物干预效果评估等科研领域，尤其为猪的血液系统疾病研究、肾脏功能探索及缺氧适应性分析提供可靠工具。样本兼容性强，可支持血清、血浆离心样本直接检测及组织匀浆预处理检测，满足不同实验设计的科研需求。
别名：

EPOErythropoietin ELISA Kit
缩写：

EPO
Uniprot No.：

P49157
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

1 mIU/mL-40 mIU/mL
灵敏度：

0.5 mIU/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig EPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	83-91
1:2	Average %	88
	Range %	84-93
1:4	Average %	97
	Range %	95-101
1:8	Average %	100
	Range %	97-103

回收率：

The recovery of pig EPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	95-102
EDTA plasma (n=4)	93	90-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3.
基因功能参考文献：
1. EPO might play a role as a survival factor or as a mitogen in developing cartilage tissue. PMID: 19908127
2. Pyruvate-fortified cardioplegia evokes myocardial erythropoietin signaling in swine undergoing cardiopulmonary bypass. PMID: 19767525
亚细胞定位：

Secreted.
蛋白家族：

EPO/TPO family
组织特异性：

Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.
数据库链接：

KEGG: ssc:397249

STRING: 9823.ENSSSCP00000008194

UniGene: Ssc.15896