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Pig Cystatin C,Cys-C ELISA Kit

  • 中文名称:
    猪半胱氨酸蛋白酶抑制剂/胱抑素C(Cys-C)酶联免疫试剂盒
  • 货号:
    CSB-E13282p
  • 规格:
    96T/48T
  • 价格:
    ¥3900/¥2500
  • 其他:

产品详情

  • 产品描述:
    猪半胱氨酸蛋白酶抑制剂/胱抑素C(Cys-C)酶联免疫试剂盒(CSB-E13282p)为竞争法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的CST3含量。CST3即半胱氨酸蛋白酶抑制剂C,在人体中广泛表达。它可抑制半胱氨酸蛋白酶的活性。在科研领域,其与神经系统疾病、肾脏疾病等方面研究较多,比如在神经退行性疾病中,CST3水平变化可能参与疾病发生发展机制。试剂盒检测范围为0.78 μg/mL-50 μg/mL,支持科研人员开展猪模型相关研究,如肾脏功能评估、代谢性疾病机制探索或药物干预效果验证等方向,尤其适用于动物实验样本中Cys-C表达水平的动态监测,为畜牧兽医学、比较医学及基础病理学研究提供可靠工具本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 缩写:
  • Uniprot No.:
  • 种属:
    Sus scrofa (Pig)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    0.78 μg/mL-50 μg/mL
  • 灵敏度:
    0.2 μg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Cardiovascular
  • 测定原理:
    quantitative
  • 测定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of pig Cys-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 86  
    Range % 82-92  
    1:2 Average % 93  
    Range % 89-97  
    1:4 Average % 92  
    Range % 84-96  
    1:8 Average % 95  
    Range % 90-100  
  • 回收率:
    The recovery of pig Cys-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 97 9-103  
    EDTA plasma (n=4) 93 89-96  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/ml OD1 OD2 Average    
    50 0.202 0.205 0.204    
    25 0.305 0.307 0.306    
    12.5 0.446 0.431 0.439    
    6.25 0.646 0.658 0.652    
    3.12 0.851 0.863 0.857    
    1.56 1.582 1.497 1.540    
    0.78 2.045 1.973 2.009    
    0 2.758 2.604 2.681    
  • 数据处理:
  • 货期:
    3-5 working days

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