Pig Corticoliberin(CRH) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

猪促肾上腺皮质素释放素(CRH)酶联免疫试剂盒（CSB-EL005963PI）为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CRH含量。CRH（促肾上腺皮质激素释放激素）是一种重要的神经调节物质，主要作用是促进腺垂体合成与释放促肾上腺皮质激素（ACTH）。近年来，研究发现CRH在慢性疼痛诱发的焦虑和抑郁中发挥关键作用，其调控痛相关抑郁的神经环路机制已得到揭示。试剂盒检测范围为100 pg/mL-1600 pg/mL，适用于基础科研领域，例如探究猪在环境压力、代谢异常或特定病理状态下CRH的表达规律，亦可应用于神经生物学、动物生理学及药理学研究中激素调控网络的解析本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

CRHCorticoliberin ELISA kit; Corticotropin-releasing factor ELISA kit; CRF ELISA kit; Corticotropin-releasing hormone ELISA kit
缩写：

CRH
Uniprot No.：

P06296
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

100 pg/mL-1600 pg/mL
灵敏度：

100 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Neuroscience
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig CRH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-95
1:2	Average %	95
	Range %	92-101
1:4	Average %	82
	Range %	80-83
1:8	Average %	88
	Range %	85-92

回收率：

The recovery of pig CRH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	87-97
EDTA plasma (n=4)	94	90-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Hormone regulating the release of corticotropin from pituitary gland. Induces NLRP6 in intestinal epithelial cells, hence may influence gut microbiota profile.
基因功能参考文献：
1. Evidence is provided for porcine corticotropin releasing hormone (CRH) as a quantitative trait locus (QTL) affecting growth and body composition. (corticotropin releasing hormone; CRH; CRF) PMID: 16483545
2. CRH inhibits local progesterone production from luteal cells in swine corpus lutem. PMID: 20431270
亚细胞定位：

Secreted.
蛋白家族：

Sauvagine/corticotropin-releasing factor/urotensin I family
组织特异性：

Produced by the hypothalamus.
数据库链接：

KEGG: ssc:100127468

STRING: 9823.ENSSSCP00000006626

UniGene: Ssc.69887