Pig Carboxypeptidase B(CPB1) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

猪羧肽酶B(CPB1)酶联免疫试剂盒（CSB-EL005883PI）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CPB1含量。CPB1，全称羧肽酶B1，是一种胰腺特异性蛋白，作为急性胰腺炎和胰腺移植排斥反应的血清标志物。研究显示，CPB1在胰腺癌全切术后复发和/或转移中表达上调，与疾病进展密切相关，可作为诊断和治疗靶点。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于科研领域对猪模型消化系统功能、营养代谢调控及相关疾病机制的探索。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

CPB1 ELISA Kit; CPBCarboxypeptidase B ELISA Kit; EC 3.4.17.2 ELISA Kit
缩写：

CPB1
Uniprot No.：

P09955
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.312 ng/mL-20 ng/mL
灵敏度：

0.078 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig CPB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	82-95
1:2	Average %	92
	Range %	85-100
1:4	Average %	94
	Range %	86-102
1:8	Average %	95
	Range %	90-100

回收率：

The recovery of pig CPB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-99
EDTA plasma (n=4)	95	90-101

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

基因功能参考文献：
1. Its catalytically important Tyr248 residue is oriented and hydrogen-bonded to solvent water molecules, and locates the furthest away from the catalytic zinc ion as compared to previous structures. PMID: 19802820
亚细胞定位：

Secreted. Zymogen granule lumen.
蛋白家族：

Peptidase M14 family
数据库链接：

KEGG: ssc:397341

STRING: 9823.ENSSSCP00000012464

UniGene: Ssc.16048