Pig Angiopoietin 2,ANG-2 ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO试剂盒采用双抗体夹心法定量检测猪血清、血浆及组织匀浆样本中的血管生成素2（ANG-2），该靶标属于血管生成素家族成员，通过调控血管内皮细胞功能参与血管生成、炎症反应等病理生理过程，是研究心血管疾病、肿瘤血管微环境及炎症机制的重要生物标志物。试剂盒检测范围覆盖0.94-60 ng/mL，最低检测限为0.94 ng/mL，灵敏度达0.23 ng/mL，实验全程仅需3小时，样本用量为100 μL。试剂盒包含预包被96孔板（12条×8孔）、标准品、检测抗体及HRP标记链霉亲和素等核心组分，标准品提供浓度梯度校准，配套终止液和洗涤缓冲液确保操作稳定性。适用于科研场景下猪模型的组织病理分析、血清/血浆循环标志物动态监测，或药物干预后血管生成相关通路的机制研究。试剂盒组分可于-20℃长期保存，开封后板条需在4℃干燥环境下存放，建议首次使用前进行标准曲线验证以获得最佳实验结果。
别名：

ANGPT2Angiopoietin-2 ELISA kit; ANG-2 ELISA kit
缩写：

ANGPT2
Uniprot No.：

Q9BDY7
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.94 ng/mL-60 ng/mL
灵敏度：

0.235 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig ANG-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	83-89
1:2	Average %	99
	Range %	96-102
1:4	Average %	87
	Range %	84-90
1:8	Average %	97
	Range %	91-103

回收率：

The recovery of pig ANG-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-92
EDTA plasma (n=4)	93	87-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating angiogenic signals mediated by ANGPT1. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.
基因功能参考文献：
1. expression of angiopoietin 2 in the porcine metanephric kidney was observed in the podocytes of early developing glomeruli, but not in the cells near the glomerular hilus PMID: 20598355
2. ANGPT2 remained upregulated during maturation of glomeruli, which may be explained by the continuous growth of the glomeruli, as observed by stereological examination. PMID: 20713984
亚细胞定位：

Secreted.
数据库链接：

KEGG: ssc:396730

STRING: 9823.ENSSSCP00000016694

UniGene: Ssc.240