Pig Angiogenin(ANG) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO猪血管生长素（ANG）酶联免疫检测试剂盒，采用竞争法定量检测血清、血浆及组织匀浆样本中的ANG含量。猪ANG是一种参与血管生成调控的关键蛋白，在组织修复、胚胎发育及病理状态下的血管新生过程中发挥重要作用。本试剂盒检测灵敏度达94 pg/mL，线性检测范围为94-6000 pg/mL，通过预包被特异性抗体实现靶标分子的精准捕获，配合优化的反应体系可有效排除样本基质干扰。实验操作全程约需3.5小时，适用于心血管疾病模型研究、再生医学实验、炎症相关血管病理分析等科研场景，特别在猪源样本的血管生成机制探索中具有重要应用价值。试剂盒包含预包被板、标准品、检测抗体及全套实验缓冲液，建议使用新鲜样本或-80℃保存的样本进行检测，避免反复冻融影响检测准确性。
别名：

ANGAngiogenin ELISA kit; EC 3.1.27.- ELISA kit; Ribonuclease 5 ELISA kit; RNase 5 ELISA kit
缩写：

ANG
Uniprot No.：

P31346
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates
检测范围：

94 pg/mL-6000 pg/mL
灵敏度：

47 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig ANG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	94
	Range %	90-98
1:200	Average %	96
	Range %	92-99
1:400	Average %	103
	Range %	97-107
1:800	Average %	84
	Range %	80-88

回收率：

The recovery of pig ANG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	98	94-102

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Stimulates ribosomal RNA synthesis including that containing the initiation site sequences of 45S rRNA. Cleaves tRNA within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs) which inhibit protein synthesis and triggers the assembly of stress granules (SGs). Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo.
亚细胞定位：

Cytoplasmic vesicle, secretory vesicle lumen. Secreted. Nucleus, nucleolus.
蛋白家族：

Pancreatic ribonuclease family
数据库链接：

STRING: 9823.ENSSSCP00000020122

UniGene: Ssc.11079