Pig Alpha-fetoprotein(AFP) ELISA kit

Pig Alpha-fetoprotein(AFP) ELISA kit

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中文名称：

猪甲胎蛋白(AFP)酶联免疫试剂盒
货号：

CSB-EL001421PI
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO猪甲胎蛋白(AFP)酶联免疫检测试剂盒，采用双抗体夹心法定量分析血清、血浆、组织匀浆及细胞培养上清液中的AFP含量。甲胎蛋白作为一种重要的胚胎发育相关糖蛋白，在胎儿期肝脏和卵黄囊中高表达，出生后水平显著下降，但在肝癌、生殖细胞肿瘤等病理状态下会重新升高，因此成为肝脏功能异常及肿瘤研究的重要生物标志物。本试剂盒检测灵敏度为3.12 ng/mL，线性检测范围覆盖3.12-200 ng/mL，可精准检测低丰度样本，适用于猪模型研究中肝脏疾病机制探索、肿瘤发生发展监测、生殖生理学研究等科研领域。试剂盒包含预包被捕获抗体的酶标板、生物素标记检测抗体及标准品等核心组分，操作流程经过严格优化，支持血清等复杂样本的直接检测，实验结果可通过酶标仪在450 nm波长下读取吸光度值进行分析。该产品为科研人员提供稳定可靠的定量检测方案，满足体外实验中对AFP蛋白表达水平动态变化的精准追踪需求。
别名：

AFPAlpha-fetoprotein ELISA Kit; Alpha-1-fetoprotein ELISA Kit; Alpha-fetoglobulin ELISA Kit
缩写：

AFP
Uniprot No.：

Q8MJ76
种属：

Sus scrofa (Pig)
样本类型：

serum, plasma, tissue homogenates, cell culture supernates
检测范围：

3.12 ng/mL-200 ng/mL
灵敏度：

0.78 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cancer
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig AFP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	88-102
1:2	Average %	92
	Range %	87-96
1:4	Average %	88
	Range %	82-94
1:8	Average %	97
	Range %	92-102

回收率：

The recovery of pig AFP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	84	80-88
EDTA plasma (n=4)	91	86-95

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
200	2.416	2.352	2.384	2.289
100	1.683	1.661	1.672	1.577
50	0.945	0.904	0.925	0.830
25	0.492	0.459	0.476	0.381
12.5	0.302	0.287	0.295	0.200
6.25	0.214	0.209	0.212	0.117
3.12	0.151	0.145	0.148	0.053
0	0.098	0.092	0.095

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin.
亚细胞定位：

Secreted.
蛋白家族：

ALB/AFP/VDB family
组织特异性：

Plasma. Synthesized by the fetal liver and yolk sac.
数据库链接：

KEGG: ssc:397586

STRING: 9823.ENSSSCP00000009550

UniGene: Ssc.7131

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.