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Pig Albumin (Alb) ELISA Kit

  • 中文名称:
    猪白蛋白酶联免疫试剂盒
  • 货号:
    CSB-E16207p
  • 规格:
    96T/48T
  • 价格:
    ¥3600/¥2500
  • 其他:

产品详情

  • 产品描述:
    CUSABIO猪白蛋白酶联免疫试剂盒(货号:CSB-E16207p),基于竞争法原理实现定量检测,适用于血清、血浆及尿液样本中猪蛋白含量的精确分析。猪蛋白作为重要的生物大分子,在动物生理代谢、免疫应答及生物医学研究中具有重要价值,其定量检测可为猪源生物制品的质量控制、营养代谢研究及疾病模型建立提供关键数据支持。本试剂盒检测灵敏度达0.03 μg/mL,线性范围覆盖0.03-7000 μg/mL,通过预包被特异性抗体与样本中目标蛋白及酶标记物的竞争性结合反应,结合显色体系实现定量检测,数据可通过标准曲线进行可靠换算。该产品广泛应用于农业科学领域猪只健康状态监测、生物制药中猪源成分残留检测、食品工业原料溯源分析,以及基础科研中猪蛋白代谢通路研究、肾脏功能评估等场景。操作流程包含样本预处理、反应孵育、洗涤及显色步骤,实验全程约需3.5小时,配套提供标准品、检测抗体及优化缓冲体系,确保实验稳定性和重复性,为科研工作者提供高效精准的检测解决方案。
  • 别名:
    ALBSerum albumin ELISA Kit
  • 缩写:
  • Uniprot No.:
  • 种属:
    Sus scrofa (Pig)
  • 样本类型:
    serum, plasma, urine
  • 检测范围:
    0.03 μg/mL-7000 μg/mL
  • 灵敏度:
    0.03 μg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Metabolism
  • 测定原理:
    quantitative
  • 测定方法:
    Competitive
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of pig albumin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:100 Average % 90  
    Range % 87-95  
    1:200 Average % 93  
    Range % 90-100  
    1:400 Average % 90  
    Range % 85-93  
    1:800 Average % 89  
    Range % 82-96  
  • 回收率:
    The recovery of pig albumin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 93-101  
    EDTA plasma (n=4) 93 89-100  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/ml OD1 OD2 Average    
    7000 0.123 0.128 0.126    
    875 0.327 0.311 0.319    
    109.4 0.612 0.623 0.618    
    13.7 0.837 0.845 0.841    
    1.7 0.950 0.967 0.959    
    0.2 1.236 1.221 1.229    
    0.03 1.493 1.518 1.506    
    0 1.844 1.904 1.874    
  • 数据处理:
  • 货期:
    3-5 working days

产品评价

靶点详情

  • 最新研究进展:
        白蛋白(ALB)是一种血浆蛋白,具有多种生理功能,如维持血容量、调节血液渗透压、输送物质等。近期的研究发现,ALB在多种疾病中起到关键作用。
        在COVID-19疫情中,患者的ALB水平通常下降,并与严重程度呈正相关,因此ALB可能作为预后生物标志物有潜在价值。
        在肾病中,ALB是最常见的蛋白质丢失之一,其下降可能导致肾脏损伤和慢性肾脏病的进展。因此,ALB已成为评估肾脏健康的重要指标。
        在癌症中,ALB水平下降与肿瘤发生和恶化有关,因此ALB可能是肿瘤预后评估的潜在生物标志物之一。
        在营养方面,ALB水平可以用作评估营养状况的指标,其下降通常与营养不良相关。
        综上所述,ALB在不同疾病中发挥着重要作用,并有望成为评估患者预后和健康状况的重要指标。
  • 功能:
    Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma. Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner. The shared binding site between zinc and calcium at residue Asp-272 suggests a crosstalk between zinc and calcium transport in the blood. The rank order of affinity is zinc > calcium > magnesium. Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli. Does not prevent iron uptake by the bacterial siderophore aerobactin.
  • 亚细胞定位:
    Secreted.
  • 蛋白家族:
    ALB/AFP/VDB family
  • 组织特异性:
    Plasma.
  • 数据库链接: