New Delhi metallo-beta-lactamase-1 (NDM-1) ELISA kit

产品详情

产品描述：

新德里超级细菌金属β-内酰胺酶-1(NDM-1)酶联免疫试剂盒（CSB-E14924）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、尿液样本中的NDM-1含量。NDM - 1即新德里金属β - 内酰胺酶 - 1，是超级细菌产生的一种耐药酶。背景上，它的出现使细菌耐药性问题更严峻。研究机制方面，它能水解多种β - 内酰胺类抗生素，让抗生素失去抗菌活性，增加了治疗细菌感染的难度，是当前研究热点。试剂盒检测范围为6.25 ng/mL- 400 ng/mL，适用于科研领域对细菌耐药机制研究、抗生素治疗效果评估以及耐药菌株的流行病学监测，为实验室开展NDM - 1相关分子特性分析、耐药基因传播规律研究提供可靠工具。本试剂盒检测数据可支持微生物学、药理学及感染性疾病领域的学术研究，助力新型抗菌药物开发及耐药性防控策略制定。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

NDM-1
样本类型：

serum, plasma, urine samples infected by New Delhi super bacteria
检测范围：

6.25 ng/mL- 400 ng/mL
灵敏度：

3.12 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of NDM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	85-103
1:2	Average %	92
	Range %	82-99
1:4	Average %	93
	Range %	87-97
1:8	Average %	95
	Range %	89-103

回收率：

The recovery of NDM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-96
EDTA plasma (n=4)	96	87-102

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.